重组人VEGF基因在游离组织移植中作用的实验研究  

Experimental study on flaps, grafts and free autologous pearl fat transplantation with rhVEGF gene in rats

作　　者：刘嘉锋[1] 钟文惠[2] 张一鸣[1] 易传勋[1] 刘宇兰[1] 聂祝锋[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院整形外科,湖北武汉430022 [2]武汉科技大学附属医院整形外科,湖北武汉430000

出　　处：《中国美容医学》2008年第4期540-543,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:研究重组人血管内皮生长因子(VEGF)基因治疗皮瓣、皮片及游离颗粒脂肪移植存活的影响。方法:将带有PCD-VEGF165的大肠杆菌接种到LB培养基中,通过碱分裂法制备PCD-VEGF165质粒,再用反蒸发法将质粒包埋于脂质体中。将108只雄性SD大鼠均分为三组,每组36只,每组再按注射的成分不同分三小组,分别为PCD-VEGF165目的基因组、PCD空白质粒组和生理盐水组,各组分别注入目的基因、空白质粒和生理盐水后在不同时间点计算组织成活率,同时取标本做成石蜡块行常规染色及免疫组化染色,检测VEGF165的表达及血管生长情况。结果:目的基因组的皮瓣及皮片成活面积较空白质粒和生理盐水组明显大(P<0.01),游离脂肪重量减少的程度亦显著小于空白质粒组和生理盐水组(P<0.05),三种移植组织中目的基因组VEGF的表达及微血管密度均显著高于其他两对照组(P<0.01),目的基因组的血管直径明显小于对照组(P<0.05),空白质粒组及生理盐水组差异无显著性意义。结论:注射转VEGF基因的质粒后可在组织中表达丰富的VEGF,诱导新血管的形成,增加血流灌注,并减少移植组织的吸收,最终促进移植皮瓣、皮片及脂肪的存活。Objective To investigate the effects of rhVEGF on flaps, grafts and free autologous pearl fat grafts in rats. Methods One hundred and eight Sprague-Dawley rats were divided into three troops： the troop of flaps, the troop of grafts and the troop of fat with thirty six of each. Each troops were divided into three groups： Injected with the plasmid DNA mediated cDNA encoding the 165-amino acid isoform of VEGF as the experiment group, the blank plasmid DNA as the negative control group and the normal saline as the frank control group. After injection the survivable ratio within the different tissues in each time was measured and specimens were harvested for immunohistologic test. Results The tissue survivable proportion of grafts and flaps in the PCD-VEGF groups were larger than the other two two control groups （P〈0.01）. The weights of the VEGF group in the fat troops were significantly reduced compared with the other two groups too （P〈0.05）.The expression of VEGF and the microvessel count in the PCD-VEGF groups were significantly higher than the other two control groups （P〈0.01）The average vessel diameter of the PCD-VEGF group was finer than the other two control groups （P〈0.05）. And the all index tested in the blank PCD groups and the normal saline groups were not different significantly （P〉0.05）. Conclusion Injected with cDNA encoding VEGF could induce the expression of VEGF and microvascular angiogenesis in flaps, grafts and fat grafting and reduce absorption in the free fat grafting. These will enhance the survival of flaps, .qrafts and free fat.

关 键 词：VEGF 皮瓣 皮片 游离脂肪 

分 类 号：Q813.1[生物学—生物工程]