膜型基质金属蛋白酶1及其诱导因子在结直肠癌中表达与预后的相关性  

High Expression of MT1-MMP and Its Inducer EMMPRIN in Human Colorectal Carcinoma and Prognosis

作　　者：刘聪[1] 岳翔[2] 刘爱国[3] 胡群[3] 周晟[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院病理研究所,武汉430030 [2]华中科技大学同济医学院附属同济医院康复科,武汉430030 [3]华中科技大学同济医学院附属同济医院儿科,武汉430030

出　　处：《华中科技大学学报（医学版）》2008年第2期161-165,279,共6页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的探讨基质金属蛋白酶诱导因子(EMMPRIN)及膜型基质金属蛋白酶1(MT1-MMP)在结直肠癌组织中的表达与肿瘤的侵袭、转移及预后的关系。方法采用逆转录聚合酶链反应(RT-PCR)检测48例结直肠癌组织及其癌旁正常组织中EMMPRIN及MT1-MMP mRNA的表达,并用免疫组化SABC法检测上述组织中EMMPRIN及MT1-MMP蛋白的表达。结果48例肿瘤组织中35例(72.9%)有EMMPRIN mRNA的表达,30例(62.5%)有MT1-MMP mRNA的表达。免疫组化染色分别有31例(64.6%)EMMPRIN和25例(52.1%)MT1-MMP呈阳性反应,但在癌旁正常组织中均未检测到EMMPRIN和MT1-MMP mRNA及蛋白的表达。EMMPRIN与MT1-MMP mRNA的相对表达强度与患者年龄、性别、肿瘤大小、浸润深度以及肿瘤类型和分化程度无关(均P>0.05),但与Duke’s分期、区域淋巴结转移和远处转移情况呈显著相关(均P<0.01),且EMMPRIN和MT1-MMP的mRNA表达呈显著正相关(r=0.728,P<0.01)。EMMPRIN mRNA阳性表达的患者平均生存期为(37.8±14.6)月,阴性患者为(54.7±11.3)月,5年生存率前者为41.2%,显著低于阴性患者的70.4%(P<0.01),EMMPRIN和MT1-MMP mRNA的阳性表达同患者的总生存率显著相关(P<0.01,P<0.05)。结论MT1-MMP及其诱导因子EMMPRIN与结直肠癌的侵袭与转移密切相关,有可能作为判断结直肠癌侵袭性的有效指标,高表达EMMPRIN和MT1-MMP可能预示着结直肠癌患者不良的预后。Objective To investigate the expression of EMMPRIN and MT1-MMP in colorectal carcinoma and its relation with metastasis, invasion and prognosis. Methods EMMPRIN and MT1-MMP mRNA and protein expression was detected by using reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemistry （IHC） respectively in 48 colorectal cancerous tissues and adjacent normal tissues. Results The positive expression rate of EMMPRIN mRNA and MT1-MMP was 72.9% and 62.5%, respectively. EMMPRIN and MT1-MMP proteins in colorectal carcinoma tissues were expressed with positive staining rate being 64.6% and 52. 1%, respectively. The expression of EMMPRIN and MT1-MMP mRNA and protein was not detectable in adjacent normal tissues. The mRNA expression of EMMPRIN and MT1-MMP in cancerous tissues was associated positively with lymph node metastasis, distant metastasis and the Duke＇s stage of disease （P〈0.01）. However, there was no correlation between the expressions of EMMPRIN and other clinicopathological parameters such as age, gender, tumor size, invasive depth and histologic pattern （P〉0.05） with a similar trend to the MT1-MMP. The expression of EMMPRIN mRNA was significantly correlated with that of MT1-MMP （r= 0. 728, P〈0.01）. The average survival time of patients with positive expression of EMMPRIN mRNA was （37.8±14.6） months and that of those with negative expression of EMMPRIN mRNA was （54.7±11.3） months, and 5-year survival rate of the former （41.2%） was significantly lower than that of the later （70.4%） （P〈0.01）. Univariate analysis revealed that the overall survival rate of patients with positive EMMPRIN and MT1-MMP expression was higher than those with negative expression （P〈0.01 and P〈0.05, respectively）. Conclusion There was a close relationship between EMMPRIN and MT1-MMP, suggesting they maybe participate in the invasion and metastasis of colorectal carcinoma. The increased expression of EMMPRIN and MT1-MMP in tumor cells is associated with poor pro

关 键 词：膜型基质金属蛋白酶1 基质金属蛋白酶诱导因子 结直肠肿瘤 预后 

分 类 号：R735.3[医药卫生—肿瘤]