慢性束缚应激对昆明小鼠脾淋巴细胞免疫功能及小鼠前胃癌移植瘤生长的影响  

Impact of Chronic Restraint Stress on Splenocyte Immunity and Growth of Mouse Forestomach Carcinoma Xenografts in Kunming Mice

作　　者：李建[1] 胡淞[1] 张才全[1] 杨海平[1] 倪春[1] 

机构地区：[1]重庆医科大学附属第一医院胃肠外科,重庆400016

出　　处：《癌症》2008年第5期471-475,共5页Chinese Journal of Cancer

摘　　要：背景与目的:近年来心理社会因素在肿瘤发生发展中的作用越来越受到关注,但其具体作用机制尚不清楚。本研究旨在探讨慢性束缚应激对昆明小鼠脾淋巴细胞免疫功能及其小鼠前胃癌(mouse forestomach carcinoma,MFC)移植瘤生长的影响,为研究心理社会因素促进肿瘤发生发展的机制提供一些依据。方法:将60只昆明小鼠按完全随机设计分为正常饲养组、单纯肿瘤组、单纯束缚组及肿瘤加束缚组,每组15只,对单纯束缚组和肿瘤加束缚组小鼠建立慢性束缚应激模型,单纯肿瘤组及肿瘤加束缚组小鼠于实验第4周时建立MFC移植瘤模型,种瘤后10d处死全部小鼠,测量移植瘤体重量,MTT法检测小鼠脾淋巴细胞增殖及对MFC细胞的生长抑制能力,酶连免疫吸附试验(enzyme-linked immunoabsorbent assay,ELISA)检测脾淋巴细胞产生IL-2的能力。结果:单纯肿瘤组瘤重(1.39±0.39)g,肿瘤加束缚组瘤重(2.10±0.52)g,与单纯肿瘤组相比,肿瘤加束缚组的MFC移植瘤生长较快,肿瘤增长率达51.08%(P<0.01);正常饲养组、单纯肿瘤组、单纯束缚组和肿瘤加束缚组小鼠的脾T淋巴细胞刺激指数分别为1.77±0.22、1.70±0.17、1.69±0.18、1.22±0.15,脾B淋巴细胞刺激指数分别为1.73±0.14、1.65±0.17、1.64±0.21、1.33±0.11,效靶比在5∶1和10∶1时小鼠脾细胞对MFC细胞的生长抑制率分别为(23.01±4.76)%、(19.47±3.70)%、(16.81±3.68)%、(7.14±5.00)%和(33.03±3.91)%、(28.34±4.58)%、(24.94±2.97)%、(13.49±7.94)%,脾细胞培养上清中IL-2含量分别为(260.03±14.96)pg/mL、(239.78±10.93)pg/mL、(238.11±13.50)pg/mL、(186.34±10.42)pg/mL,两因素方差分析显示慢性束缚应激和移植瘤均使小鼠脾淋巴细胞增殖能力、对MFC细胞的生长抑制能力及产生IL-2的能力减弱,并且两者具有交互效应,其中以慢性束缚应激的影响程度较大(P<0.01)。结论:慢性束缚应激可通过削弱小鼠的免疫功能促进MFC移植瘤生长。BACKGROUND ＆ OBJECTIVE. Recent study foundpsychosocial factors play some important roles in carcinogenesis and development of malignant tumors, but its mechanisms remain unclear. This study was to investigate the impact of chronic restraint stress on splenocyte immunity and growth of mouse forestomach carcinoma （MFC） xenografts in Kunming mice, and provide evidences for exploring the mechanisms of psychosocial factors function on malignant tumors. METHODS. A total of 60 Kunming mice were randomized into normal control group, restraint stress group, tumor-bearing group and tumor plus restraint stress group; each group contained 15 mice. Chronic restraint stress models were established in restraint stress group and tumor plus restraint stress group; MFC xenograft models were established in tumor-bearing group and tumor plus restraint stress group 4 weeks later. Mice were killed 10 days after inoculation of MFC cells. The weight of MFC xenografts were measured. The proliferation and cytotoxicity of splenocytes were detected by MTT assay. The level of interleukin-2 （IL-2） in splenocyte culture supernants was detected by enzyme-linked immunoabsorbent assay （ELISA）. RESULTS. The weight of MFC xenografts was （1.39±0.39） g in tumor-bearing group and （2.10±0.52） g in tumor plus restraint stress group; MFC xenografts grew faster in tumor plus restraint stress group than in tumor-bearing group （P〈0.01）, with a tumor growth rate of 51.08%. In normal control group, restraint stress group, tumor-bearing group, and tumor plus restraint stress group, the stimulus indexes （SI） of T lymphocytes were 1.77±0.22, 1.70±0.17, 1.69±0.18, and 1.22±0.15, respectively; the SI of B lymphocytes were 1.73±0.14, 1.65± 0.17, 1.64±0,21, and 1.33±0.11, respectively; the inhibition rate of MFC cell proliferation were （23.01 ±4.76）%, （19.47±3.70）%, （16.81 ±3.68）%, and （7.14±5.00）%, respectively, when the effector/target ratio was 5：1 and （33.03±3.91）%, （28.34±4.58）%, （24.94

关 键 词：慢性束缚应激 免疫功能 肿瘤 前胃癌移植瘤 小鼠 

分 类 号：R735.2[医药卫生—肿瘤]