金水乌鸡PRL基因多态性研究  

Study on the PRL Gene Polymorphism of Jinshui Silk Fowl

作　　者：潘爱銮[1] 皮劲松[1] 杜金平[1] 梁振华[1] 申杰[1] 蒲跃进[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,湖北武汉430209

出　　处：《安徽农业科学》2008年第11期4457-4458,4597,共3页Journal of Anhui Agricultural Sciences

基　　金：湖北省自然科学基金项目(2005ABA194);湖北省动物胚胎工程及分子育种重点实验室项目(2007A0701)

摘　　要：[目的]研究金水乌鸡PRL基因多态性,为金水乌鸡的保种和持续选育与提高积累分子基础数据。[方法]采用PCR-RFLP方法研究金水乌鸡白羽丝毛系165只乌鸡PRL基因多态性,分别采用HaeⅢ和HhaⅠ2种内切酶对各DNA样品的PCR产物进行单酶切,分析基因型与产蛋数的相关。[结果]金水乌鸡PRL基因的PCR产物的HhaⅠ酶切片段未出现多态性。金水乌鸡PRL基因的PCR产物的HaeⅢ酶切片段呈现RFLP多态性,有AA、AB、BB3种基因型,A基因频率较高。相关分析结果表明,不同基因型的产蛋数差异不显著。[结论]A基因可能是影响产蛋数的优势基因。[Objective] The study aimed to analyze the PRL gene polymorphism of Jinshui silk fowl, and accumulating molecule basic data for protecting genetic resources and breeding afterwards and improving performance. [Method] The Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms （PCR-RFLPs） of PRL gene in 165 Jinshui silk fowls were investigated. Two restrictions of Hha Ⅰ, Hoe Ⅲwere used to digest the production of PCR respectively. [Result] It was demonstrated that 3 different genotypes of AA,AB,BB had been detected for Hoe Ⅲ site. The frequency of A gene was greatly higher than that of B gene. The result of relation test showed that there was no significant difference for laying performance between the chicken of different genotypes. [Conclusion] A gene could be the superiority gene of effecting the laying performance.

关 键 词：金永乌鸡 PRL基因 多态性 

分 类 号：S831[农业科学—畜牧学]