交感神经递质及其受体在小鼠血吸虫肝纤维化中的作用  

The effects of sympathetic and adrenergic receptors on liver fibrosis in murine schistosomiasis

作　　者：段瑞娴[1] 唐望先[2] 吴翠环[3] 刘红艳[2] 高啸[2] 郭燕[2] 程勇卫[1] 杨玉珍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院消化内科,武汉430030 [2]华中科技大学同济医学院附属同济医院肝病研究所 [3]华中科技大学同济医学院病理教研室

出　　处：《中华肝脏病杂志》2008年第5期352-354,共3页Chinese Journal of Hepatology

基　　金：国家自然科学基金（30571627）

摘　　要：目的研究交感神经递质及其受体在正常小鼠及血吸虫病肝纤维化小鼠体内的变化情况。方法应用腹部敷贴尾蚴法制备血吸虫病小鼠模型。30只昆明小鼠随机分为正常对照组和肝纤维化模型组。采用HE和Van Gieson染色，光镜观察肝组织病理学改变及纤维化程度，免疫荧光组织化学染色结合激光共聚焦扫描检测肝组织中α1A、β2肾上腺素能受体表达情况，同时用高效液相色谱电化学法检测血浆中去甲。肾上腺素和多巴胺的水平。结果正常组α1A、β2肾上腺素能受体散在表达于肝细胞胞质和肝窦内，模型组在门静脉和虫卵结节周围肝细胞胞质中阳性表达明显增加（α1A受体的平均灰度值分别为18．28±7．56、30．53±8．88，β2受体的平均灰度值分别为28．67±6．42、42．29±4．56，t值分别为2．888和6．648，P值均〈0．05）；血吸虫肝纤维化小鼠和正常小鼠去甲。肾上腺素含量分别为（7．83±4．36）、（2．72±0．94）ng／ml，多巴胺的含量分别为（6．97±4．33）、（0．74±0．34）ng／ml，t值分别为3．372和4．428，P值均〈0．05。结论交感神经递质及其受体表达的上调可能是血吸虫肝纤维化发展的作用机制之一。Objective To investigate the effects of sympathetic neurotransmitters and adrenergic receptors on liver fibrosis in murine schistosomiasis. Methods Mice were infestated with schistosoma by means of pasting cercariae on their abdomens. Thirty mice were randomly divided into a control group and a model group. Hematoxylin eosin and Van Gieson staining were used to view the histopathology of their livers. Immunofluorescence histochemistry and laser scanning confocal fluorescence microscopy were used to measure the α 1A and β2 adrenergic receptors in livers of the two groups of mice. High performance liquid chromatography-electrochemical detector （HPLC-ECD） was used to determine the concentration of norepinephrine （NE） and dopamine （DA） in the plasma of the mice. Results Immunofluorescence histochemistry showed that α 1A and β2 receptors were present in hepatocytes and hepatic sinusoids of the livers of the mice of the two groups, but there were many more in the livers of the schistosoma infected mice （t = -2.888; t = -6.648） （P 〈 0.05）. The results of HPLC-ECD showed that the levels of NE and DA in the model group were higher than those of the control group （t = -3.372; t = -4.428） （P 〈 0.05）. Conclusion Sympathetic neurotransmitters and adrenergic receptors may participate in liver fibrogenesis in mice infected with schistosoma.

关 键 词：血吸虫病 肝纤维化 去甲肾上腺素 受体  肾上腺素能 多巴胺 

分 类 号：R575.2[医药卫生—消化系统] R532.2[医药卫生—内科学]