高强度聚焦超声对裸鼠皮下人胶质瘤的治疗作用  

Treatment of human neurogliocytoma of in a nude mouse model using high intensity focused ultrasound

作　　者：肖文峰[1] 霍钢[1] 郑履平[1] 唐文渊[1] 孙晓川[1] 支兴刚[1] 李发琪[2] 

机构地区：[1]重庆医科大学附属第一医院神经外科,重庆400016 [2]重庆医科大学生物医学工程系

出　　处：《中国神经精神疾病杂志》2008年第4期208-211,共4页Chinese Journal of Nervous and Mental Diseases

基　　金：重庆市卫生局资助项目(编号:04-2-196)

摘　　要：目的通过研究高强度聚焦超声（high intensity focused ultrasound，HIFU）对裸鼠皮下人胶质瘤模型作用后的病理转归，探讨其治疗胶质瘤的有效性和安全性。方法选取24只裸鼠，建立裸鼠皮下人胶质瘤模型，随机分为对照组和实验组。用频率9．7MHz，焦距4．5mm，声强2500W／cm^2的HIFU连续作用20s，于急性期（〈3d）、亚急性期（4～14d）、慢性期（15～30d）观察各组肿瘤的生长情况；行2，3，5-三苯基氯化四氮唑（triphenyl tetrazolium choloride，TFC）染色、组织学切片及电镜检查。结果24只裸鼠全部成瘤，接种20d后肿瘤体积为（166．56±9．23）mm^3，在HIFU作用7d后，实验组肿瘤体积倍增时间（tumor volume doubling time，TD）与对照组差异有统计学意义（P〈0．05）。TFC染色显示胶质瘤的生物学焦域为（66．12±2．52）mm^3。HE染色示照射区即刻就发生凝固性坏死，并随观察时间的延长坏死越明显；在亚急性期和慢性期可以观察到明显的交界区形成，且在照射区、交界区之间形成锐利的边界。电镜示细胞超微结构出现典型的凝固性坏死改变。结论在本试验设定参数及声学剂量情况下，HIFU能够准确、完全杀灭照射区胶质瘤，有望成为治疗胶质瘤的新途径。Objective To explore the feasibility of high intensity focused ultrasound （HIFU） as a therapy for neurogliocytoma by assessing tumor regression of subcutaneous human neurogliocytoma xenografted in nude mice after ablation. Methods Twenty four nude mice bearing subcutaneous human neurogliocytoma xenografts were divided into control and experimental group randomly. An extracorporeal HIFU with 9.7 MHz transducer（the focal length of 4. 5 mm and focal intensity 2500 W/cm^2 ） was used in the experiments. The HIFU treated animals received 20s consecutive ablation. Triphenyl tetrazolium choloride （TTC） staining, HE staining, and electron microscope were performed to evaluate the growth of subcutaneous human neurogliocytoma at acute （ 〈 3 d）, subacute （4 - 14 d） and chronic phase （15 - 30 d） after ablation. Results All of 24 nude mice were successfully inoculated with SHG-44. After 20 days, subcutaneous human neurogliocytoma was （166. 56 ±9. 23 ） mm^3. The tumor volume doubling time （TD） was significantly different between control and experimental group （P 〈 0.05 ） and biological focal region （BFR） detected by TIC was （66. 12 ± 2. 52） mm^3. HE staining showed that typical coagulation necrosis was instantly found at exposure areas after ablation, which became more apparent over time and extended to marginal zone in subacute stage. The boundary between the exposure coverage and marginal zone became distinct in subacute stage and cbronic phase. Typical coagulation necrosis of cell uhramicrostructure was also lbserved by electron microscope. Conclusions HIFU at parameters and dose used in this study can precisely and completely ablate neurogliocytoma at exposure coverage. HIFU might become a new and selective way for therapy of neurogliocytoma.

关 键 词：高强度聚焦超声 胶质瘤 有效性 交界区 

分 类 号：R739.4[医药卫生—肿瘤]