双氢麦角碱对血管性痴呆小鼠海马乙酰胆碱酯酶活性变化的影响  

Effect of dihydroergocriptine on the activities of acetylcholinesterase in hippocampus of mice with vascular dementia

作　　者：吕佩源[1] 靳玮[1] 冯志山[2] 何春年[3] 翟金萍[3] 

机构地区：[1]河北省人民医院神经内科,050051 [2]河北省人民医院生化室,050051 [3]河北省人民医院病理科,050051

出　　处：《神经疾病与精神卫生》2008年第2期88-90,共3页Journal of Neuroscience and Mental Health

基　　金：河北省自然科学基金资助项目(编号:301415)

摘　　要：目的探讨双氢麦角碱对血管性痴呆(VD)小鼠海马组织乙酰胆碱酯酶(AchE)活性变化的影响。方法将75只小鼠随机分为假手术组、模型组和药物组。药物组和模型组采用双侧颈总动脉反复缺血再灌注法制备VD模型,术后第29天和第30天测试学习记忆成绩,术后第30天检测海马AchE含量,并研究双氢麦角碱对二者的影响。结果(1)模型组小鼠学习记忆成绩较假手术组明显下降(P<0.01),治疗组小鼠学习记忆成绩明显优于模型组小鼠(P<0.01);(2)模型组小鼠海马AchE活性较假手术组明显降低(P<0.01),治疗组小鼠海马AchE活性显著高于模型组小鼠(P<0.05)。结论双氢麦角碱能改善VD小鼠的学习记忆功能,提高海马组织内的AchE活性。Objective To study the effects of dihydroergocripune on the activities of acetylcholinesterase （AchE） in hippocampus of mice with vascular dementia （VD）. Methods 75 kunming mice were randomly divided into model group, dihydroergocriptine treatment group and sham-operated group. In model group and dihydroergocriptine group, the VD models were established by three repeated times ischemia- reperfusion through the ligation of the bilateral common carotid arteries. The abilities of learning and memory of mice were investigated by step down test and water maze test to all animals. The activities of AchE in hippocampus were measured on the 30th day after the operauon in 3 groups of mice. Results （1）The grades of learning and memory of mice in model group were lower than the grades in shame group （P 〈 0.01）. Compared with model group, the grades of learning and memory of mice in drug treatment group were improved significantly （P 〈 0.01）. （2）The activities of AchE of hippocampus in model group was less than in sham group （P 〈 0.01）, however, which was evidently increased in drug group compared with model group （P 〈 0.05）. Conclusions Dihydroergocriptine can improve learning and memory abilities in mice models of ischemia reperfusion injury and enhance the activities of AchE in hippocampus of those mine.

关 键 词：血管性痴呆 水迷宫 跳台试验 乙酰胆碱酯酶 二氢麦角碱 

分 类 号：R749.16[医药卫生—神经病学与精神病学]