少弱畸精子综合征治疗前后精子染色质流式细胞术分析  

Changes of Sperm Chromatin in Oligo-asteno-teratozoospermia Syndrome Patients After Treated by Integrated Chinese and Western Medicine

作　　者：杜江平[1] 杨书文[2] 门金娥[3] 王霞[4] 张向阳[3] 郑海萍[3] 李艳[5] 

机构地区：[1]河北工程大学临床医学院泌尿外科,河北邯郸056029 [2]河北医科大学第二医院泌尿外科,河北石家庄050000 [3]河北工程大学临床医学院中心实验室,河北邯郸056029 [4]邯郸市妇幼保健院不孕不育科,河北邯郸056002 [5]河北峰峰集团公司总医院邯郸医院,河北邯郸056002

出　　处：《中华男科学杂志》2008年第4期334-337,共4页National Journal of Andrology

摘　　要：目的:观察少弱畸精子综合征(OAT)患者采用中西医结合治疗前后精子染色质的变化。方法:对60例OAT患者给予中西医结合治疗3个月,留取治疗前后精液标本,进行吖啶橙(AO)标记,流式细胞术分析以及激光共聚焦显微镜观察绿、黄和红色荧光精子百分率,同时进行常规分析。结果:治疗前后流式细胞术提示主群精子变化存在显著差异(P<0.01)、αt和COMPαt变化存在显著差异(P<0.05)。激光共聚焦显微镜观察时,绿色与红色两种荧光有显著差异(P<0.05),而黄色荧光无明显差异(P>0.05)。计算机辅助精子分析结果:精子密度、活动力、活动率和畸形率等均有变化。除直线运动精子密度无显著差异外(P>0.05),密度、活动率、活动力均有显著差异(P<0.05)。结论:中西医结合治疗OAT综合征可以改善精子染色质。综合流式细胞术、CASA、精子AO染色激光共聚焦显微镜观察等3种检测手段更有助于疗效判断。Objective： To observe the changes of sperm chromatin in patients with oligo-asteno-teratozoospermia （OAT） syndrome after treated by integrated Chinese and Western medicine. Methods： Sixty patients with OAT syndrome were treated by integrated Chinese and Western medicine for 3 months. Their sperm samples were collected before and after the treatment, subjected to acridine orange staining and analyzed by fluorescent microscopy, flow cytometry and sperm routine detection. Results： Significant differences were shown in the master-group sperm signals （P 〈 0.01） and αt and COMPαt （P 〈 0.05） by flow cytometry, as well as in the green and the red groups （P 〈 0.05） by fluorescent microscopy before and after the treatment. Changes in sperm concentration, mo-tility, vitality and deformity were noted after the treatment, with statistic difference between＇ pre- and post-treatment （ P 〈 0.05 ） except in forward sperm concentration. Conclusion： Treatment by integrated Chinese and Western medicine can improve sperm chromatin in patients with OAT syndrome. Flow cytometry, along with fluorescent microscopy and sperm routine detection, plays an important role in the evaluation of male infertility therapy.

关 键 词：少弱畸精子综合征 精子 染色质结构 流式细胞术 吖啶橙染色 

分 类 号：R698.1[医药卫生—泌尿科学] R21[医药卫生—外科学]