条斑紫菜中R-藻红蛋白的纯化及其α和β亚基的分离与发色团含量的测定  

PURIFICATION OF R-PHYCOERYTHRIN IN PORPHYRA YEZOENSIS AND SEPARATION, CHROMOPHORE DETERMINATION OF ITS α AND β SUBUNITS

作　　者：程凌江[1] 蒋丽金[1] 马金石[1] 

机构地区：[1]中国科学院感光化学研究所

出　　处：《海洋与湖沼》1990年第4期337-342,共6页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金

摘　　要：本文从条斑紫菜(Porphyra yezoensis)中提取了藻胆蛋白,找到了一种新的简便有效的提纯R-藻红蛋白的方法。用Bio-GelP300柱进一步纯化后,得到了高纯度的R-藻红蛋白。用Bio-Rex 70柱分离R-藻红蛋白,得到了它的α和β亚基溶液。对亚基溶液进行的一些光谱分析表明亚基溶液具有R-藻红蛋白的一些性质。本文对α和β亚基的发色团含量进行了测定:α亚基含2个藻红胆素,β亚基含3个藻红胆素和1个藻尿胆素。A new, simple and efficient method for separating R-phycoerythrin from biliproreins extracted from Porphyra yezoensis has been developed. After being purified by filtrating through Bio-Gel P300 column, the R-phycoerythrin was obtained with high purity (A560nm/A280nm = 8.1). The subunits were separated through Bio Rex 70 column in urea solution (pH = 2.5). The spectroscopic analyses of the subunit solutions manifested that the subunits were similar in some properties to R-phycoerythrin. The chromophore compositions of a and β subunits were determined to be 2 phycoerythrobilins in α subunit and 3 phycoerythrobilins and 1 phycourobilin in β subunit.

关 键 词：紫菜 R-藻红蛋白 纯化 亚基溶液 

分 类 号：Q949.292.1[生物学—植物学]