人成牙本质细胞L型钙离子通道特异性基因片段的克隆研究  

Cloning of L type calcium channel specific gene fragment from human odontoblast

作　　者：赵颖煊[1] 赵守亮[2] 唐荣银[1] 吕海鹏[1] 闫晶[1] 侯锐[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032 [2]同济大学口腔医学院,上海200172

出　　处：《牙体牙髓牙周病学杂志》2008年第4期208-211,共4页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(30471889)

摘　　要：目的:克隆人成牙本质细胞L型钙离子通道α1亚基D亚型特异性基因片段。方法:选取新鲜拔除的健康恒牙,利用牙科专用慢速切锯制备牙齿切片,采用酶消化法分离出完整的人成牙本质细胞,利用RT-PCR技术直接从人成牙本质细胞中克隆L型钙离子通道α1亚基D亚型特异性基因片段,将其亚克隆入pGM-T载体进行序列测定。结果:从人成牙本质细胞中获得452bp的特异性片段。序列分析表明,其与Gen-Bank中已登录的人L型钙离子通道α1亚基D亚型特异性基因序列一致。结论:成功地从人成牙本质细胞中克隆到人成牙本质细胞L型钙离子通道α1亚基D亚型的特异性基因序列片段。AIM： To clone L type calcium channel od D subunit specific gene fragment from human odontoblast. METHODS： Freshly extracted human permanent teeth were collected and sectioned under cooling ECS solution and then the odontoblasts were isolated through enzyme digestion. L type calcium channel α1 D subunit specific gene fragment from human odontoblasts was cloned by RT - PCR. Then the target fragment was inserted into pGM - T vector and sequenced. RESULTS：A 452 bp fragment was obtained from human odontoblasts. The sequence analysis showed that it was 99.9% homologous to human L type calcium channel α1D subunit specific gene reported by Genebank. CONCLUSION ：The specific sequence of L type calcium channel α1D subunit of human odontoblast was successfully cloned from human odontoblast.

关 键 词：人成牙本质细胞 L型钙离子通道α1亚基D亚型 酶消化法 基因克隆 

分 类 号：R780.2[医药卫生—口腔医学]