基因活化剂对紫肉甘薯根系活力的影响  

Effects of a Gene Activator on the Vigor of the Root Systems of Purple-Fleshed Sweet Potato

作　　者：汤绍虎[1] 周启贵[1] 龙云[1] 李坤培[1] 刘开全[1] 

机构地区：[1]西南大学生命科学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2008年第4期92-95,共4页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市农业综合开发办“国家农业综合开发科技示范资助项目”(2001-20)

摘　　要：基因活化剂使用浓度(A)设3个水平:375×(A1)、750×(A2)和1 500×(A3)稀释液,处理时间(B)设3个水平:浸渍苕秧基部0.5 h(B1)、1 h(B2)和2 h(B3),处理后培养在改良Hoagland培养基中,12 d后测定根系α-萘胺氧化速率、NO3-和K+吸收速率.结果表明:A1、A2和A3的α-萘胺氧化速率分别比对照(98.46μg/g.h)提高2.99,5.92和2.37倍;NO3-吸收速率分别比对照(0.23 mg/株.h)提高0.51,1.14和0.66倍;K+吸收速率分别比对照(0.12 mg/株.h)提高1.73,2.73和1.16倍.与对照相比,B1、B2和B3的α-萘胺氧化速率分别提高5.50,3.29和2.49倍;NO3-吸收速率分别提高0.60,1.09和0.63倍;K+吸收速率分别提高1.57,2.66和1.40倍.因素A、B与对照差异达极显著水平,不同水平间差异极显著(p<0.01).A2B2是最佳水平组合,即用750倍的基因活化剂浸渍苕秧基部1 h,可显著提高紫肉甘薯的根系活力,比对照α-萘胺氧化速率、NO3-和K+吸收速率分别提高5.75,1.51,3.52倍.In the experiment, three concentrations of a gene activator were used： A1, A2 and A3, which were diluted by375×（A1）, 750×（A2） or 1500×（A3）. Plants were treated for0.5 h （B1）,1h （B2） or2 h （B3）. The experimental materials dipped were cultured in liquid Hoagland medium and the oxidization of a-naphthylamine, and absorption of NO3^- and K^＋ of the treated plants were determined after 12 days. The results showed that plants with the three solution concentrations increased the oxidizing rate of a-naphthyl- amine by 2.99, 5.92 and 2.37 times, respectively, their absorption rate of NO3^- increased by 0.51, 1.14 and 0.66 times, respectively, and their absorption rate of K^＋ increased by 1.73, 2.73 and 1.16 times, respectively. Plants subjected to three different time treatments increased their oxidization of a-naphthyl- amine by 5.50, 3.29 and 2.49 times, respectively, their absorption of NO3^- increased by 0.60, 1.09 and 0.63 times, respectively, and their absorption of K^＋ increased by 1.57, 2.66 and 1.40 times, respectively. The mean differences were significant at 0.01 among different treatments and levels of factors, the combination A2B2 was the best treatment, and its oxidization of a-naphthylamine and absorption of NO3^- and K^＋ increased by 5.75, 1.51 and 3.52 times, respectively.

关 键 词：基因活化剂 紫肉甘薯 根系活力 

分 类 号：S531[农业科学—作物学]