拮抗放线菌WS-03030的分类鉴定及其抗菌成分的初步研究  

Classification of Actinomycetes WS-03030 and Its Antifungal Substances

作　　者：陈涛风[1] 王开梅[2] 万中义[2] 杨自文[2] 

机构地区：[1]中国科学院武汉病毒所,武汉430061 [2]湖北省生物农药工程研究中心

出　　处：《湖北农业科学》2008年第4期427-429,共3页Hubei Agricultural Sciences

基　　金：湖北省"十一五"重大科技攻关项目(2006AA205A01);湖北省农业科技创新中心创新团队项目资助(2006AA205A01)

摘　　要：WS-03030是从武汉地区土壤中分离到的1株具有抗真菌活性的菌株。经形态学、培养特征、生理生化特征培养观察及16SrDNA序列分析,将WS-03030鉴定为吸水链霉菌;WS-03030发酵液的乙酸乙酯粗提物经半制备高效液相及活性测定确认了产生的活性物质,活性物质通过高效液相制得的纯品对稻瘟病菌、黄色镰刀菌和小麦颖枯病菌的最低抑制浓度(MIC)仅1.562 5μg.mL-1。In screening process of new agricultural antibiotics, a aetinomycetes strain WS-03030 was isolated and identificated from a soil sample collected in Wuhan. The results showed that WS-03030 belonged to Streptomyces hygroseopieus. Its bioactive substance was isolated by ethyl acetate extraction and purified by preparative HPLC through activity-guiding method. Antifungal experiment showed that the substance＇ MIC to Pyrieularia oryzae, Fusarium eulmorum, Septoria nodorum could reach 1.562 5 μg· mL^-1.

关 键 词：农用抗生素 分类鉴定 分离纯化 抑菌活性 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]