绵羊痘病毒甘肃流行株糖蛋白基因orf118的克隆及序列分析  

Cloning and analysis of glycoprotein-encoding orf118 from sheeppox virus Gansu strain

作　　者：张冬峰[1,2] 郑亚东[1,2] 骆学农[1,2] 王晓霞[1,2] 陈轶霞[3] 李辉[1,2] 侯俊玲[1,2] 潘晓梅[1,2] 刘琼[1,2] 景志忠[1,2] 岳城[4] 才学鹏[1,2] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室 [2]中国农业科学院人兽共患病重点实验室,甘肃兰州730046 [3]西北民族大学生命科学与工程学院,甘肃兰州730046 [4]新疆农业大学动物医学院,新疆乌鲁木齐830052

出　　处：《湖南农业大学学报（自然科学版）》2008年第2期200-203,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：中国农业科学院兰州兽医研究所所长基金;兰州市城关区农业科技攻关项目(07-32)

摘　　要：从甘肃景泰疑似羊痘患病绵羊的皮肤组织中提取基因组DNA,利用PCR技术,克隆糖蛋白orf118基因.比较分析表明,该基因由516个核苷酸组成,编码171个氨基酸,相对分子质量为19500,其碱基组成极不平衡,其中A+T含量占72.48%,G+C含量仅占27.52%.甘肃景泰株orf118基因同绵羊痘病毒TU-V02127株之间的核苷酸同源性最高达99.8%,氨基酸的同源性为100%;与疙瘩皮肤病病毒肯尼亚株和山羊痘病毒G20-LKV株之间的同源性分别为96.5%和94.8%.结构预测结果显示,orf118蛋白为分泌蛋白,具有2个N键连结糖基化位点和1个跨膜区.以上结果表明,orf118蛋白在不同的绵羊痘病毒流行株之间非常保守,具有潜在的疫苗和诊断价值.Genomic DNA was extracted from the skin tissue of suspected capripox sheep in Jingtai, Gansu Province, and the glycoprotein-encoding orf118 gene was cloned by PCR. Comparison shows that the length of open reading frame of glycoprotein-encoding orf118 gene is 516 bp, coding for a 171-amino acid protein with molecular weight of approximate 19 500, and its nucleotide component showed a serious imbalance with 72.48% of A＋T and 27.52% of G＋C. The homology of nucleotide and deduced amino acid sequence between sheeppox virus ,Gansu strain and sheeppox virus TU-V02127 strain is 99.8% and 100%, respectively, orf118 of the sheeppox virus, Gansu strain shares 96.5% and 94.8% of identity with Lumpy skin disease virus and goatpox virus G20-LKV strain at a nucleotide level. Structural prediction of the deduced orf118 protein indicates that the orf118 antigen is a secretory glycoprotein with two N-linked glycosylation sites and one trans-membrane domain. Together with above results suggest that the orf118 glycoprotein is completely conserved among sheeppox field strains and has potential value for vaccine and diagnosis.

关 键 词：绵羊痘病毒 orf118基因 糖蛋白 PCR 

分 类 号：S858.26[农业科学—临床兽医学]