体外培养人皮肤鳞癌A431细胞Smad4的表达下调  

Down-regulated expression of Smad4 in A431 cell line derived from human epidermoid squamous cell carcinoma

作　　者：张斌[1] 何威[1] 李承新[2] 高天文[2] 李颖[1] 郁博[1] 杨扬[1] 

机构地区：[1]第三军医大学新桥医院皮肤科,重庆400037 [2]第四军医大学西京医院全军皮肤病研究所,西安710032

出　　处：《重庆医学》2008年第9期926-927,930,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(30371298)。

摘　　要：目的研究体外培养的人皮肤鳞癌细胞中Smad4的表达情况。方法采用反转录-实时荧光定量聚合酶链式反应及Western blotting,分别检测体外培养的人皮肤鳞癌A431细胞与人永生化角质形成细胞(HaCaT细胞)中Smad4 mRNA及其蛋白的表达水平。结果在mRNA表达水平上,A431细胞与对照HaCaT细胞相比,其2-△△CT值大于2,在蛋白表达水平上,两者差异有统计学意义(t=2.536,P<0.05),提示人皮肤鳞癌A431细胞Smad4的mRNA及其蛋白的表达水平均显著低于对照的HaCaT细胞。结论Smad4表达下调可能是鳞癌A431细胞所固有的转化生长因子β信号转导通路的异常变化之一。Objective To investigate the expression of Smad4 in carcinous keratinocytes derived from human epidermoid squamous cell carcinoma. Methods Reverse transcription quantitative real-time PCR and Western blotting were utilized to assess the expression of Smad4 in A431 cell line derived from human epidermoid squamous cell carcinoma and human immortalized keratinocytes （HaCaT ceils） respectively. Results The expression levels of Smad4 in A431 ceils were significantly down-regulated in comparison with those of HaCaT ceils. Conclusions It is possible that the down-regulated expression of Smad4 represents intrinsic alteration of TGF-β signaling pathways,which contributes to aberrant hyperplasia of tumor ceils in squamous cell carcinoma.

关 键 词：鳞状细胞癌 角质形成细胞 转化生长因子-β SMAD4 

分 类 号：R739.5[医药卫生—肿瘤]