雌激素受体β在大鼠结肠的表达及其对Cajal间质细胞、结肠肌电影响的实验研究  

Expression of ERβ in rat colon and its effect on interstitial cells of cajal colonic motility

作　　者：张广军[1] 刘宝华[1] 童卫东[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所普外科,重庆400042

出　　处：《重庆医学》2008年第9期942-943,I0001,共3页Chongqing medicine

基　　金：重庆市自然科学基金项目(2006BB5114)

摘　　要：目的探讨雌激素受体β(estrogen receptorβ,ERβ)在大鼠结肠的表达及其对结肠肌电、Cajal间质细胞(interstitialcells of Cajal,ICC)的影响。方法免疫组化观察ERβ在正常大鼠结肠的表达和分布。20只大鼠随机分为两组,实验组给予他莫昔芬(tamoxifen,TAM)灌胃,3个月后测定结肠慢波频率及振幅;免疫荧光染色观察肠壁ICC数目的变化。结果ERβ在大鼠结肠黏膜、黏膜下及肌间神经丛表达。TAM拮抗雌激素后,大鼠结肠慢波频率及振幅均明显降低(P<0.05),肌间神经丛及黏膜下神经丛ICC数目较对照组明显减少(P<0.01)。结论ERβ信号通路可能通过影响ICC,从而导致结肠动力减弱。Objective To investigate the expression of ERβ and its effect on colonic motility and interstitial ceils of Cajal in rats. Methods Immunohistochemistry methord was used to detect the distribution and expression of ERβ in rats. Frequency and amplitude of colonic slow waves were examined after the animals fed for 3 months. The number of ICC were observed by fluorescence staining. Results ERβ was detected in mucous layer, myenteric nerve plexus and submucous nerve plexus in colon. In comparison with the control group,the frequency and amplitude of slow waves of experimental group were reduced significantly （P〈0.05） ,and the number of ICC also decreased （P〈0.01）. Conclusion ERβ signaling pathway can influence the number of ICC and furthermore lead to STC.

关 键 词：雌激素受体Β CAJAL间质细胞 他莫昔芬 

分 类 号：R574.6[医药卫生—消化系统]