胃癌细胞TRAIL受体的表达与其对TRAIL敏感性的关系  

Relation between TNF-Related Apoptosis-induing Ligand Receptors Expression and TRAIL-induced Apoptosis of Gastric Adenocarcinoma

作　　者：吴萍[1] 张旭东 张林杰[1] 

机构地区：[1]安徽医科大学免疫学教研室,安徽合肥230032 [2]澳大利亚纽卡索玛特医院肿瘤与免疫实验室

出　　处：《现代生物医学进展》2008年第7期1286-1288,1298,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(编号:30572118);安徽省自然科学基金项目(编号:070413077)

摘　　要：目的:探讨胃癌细胞表面TRAIL受体表达水平及其与TRAIL敏感性的关系。方法:PI染色、流式细胞仪检测TRAIL诱导BGC-823及SGC-7901细胞的凋亡率,流式细胞仪检测细胞膜表面四种TRAIL受体-R1、R2、R3、R4的表达情况。结果:TRAIL诱导胃癌细胞凋亡具有剂量和时间依赖性,BGC-823较SGC-7901对TRAIL诱导的凋亡更敏感,TRAIL(100μg·L^(-1))作用24 h的细胞凋亡率分别是59.9%、24.3%。死亡受体TRAIL-R1/DR4、TRAIL-R2/DR5在BGC-823细胞膜表面表达的阳性率高达97.87%和99.42%,而在SGC-7901分别为7.03%和95.31%,诱骗受体TRAIL-R3/DcR1、TRAIL-R4/DcR2在两株细胞膜表面极少表达。结论:胃癌细胞对TRAIL诱导凋亡的敏感性差异可能与细胞膜表面死亡受体有关,尤其与DR4的表达有关。Objective： To investigate the expression of TRAIL receptors on the cell surface and its relation to TRAIL-induced apoptosis of gastric adenocarcinoma cell lines. Methods： Apoptotic cells were determined by the propidium iodide method using flow cytometry. TRAIL-R1,R2,R3,R4 cell surface expression were evaluated by flow cytometry. Results： TRAIL- induced apoptosis of gastric adenocarcinoma cells was in a dose- and time-dependent manner. BGC-823 was more sensitive to TRAIL-induced apoptosis than SGC-7901. The rates of apoptotic cells were 59.9 % and 24.3 % after treatment with TRAIL （ 100 μg·L^-1） for 24 h. The cell surface expression of death receptors, TRAIL-R1 and -R2 were 97.87 % and 99.42 % on the BGC-823 cell line, while which were 7.03 % and 95.31% on the SGC-7901 cell line. The levels of cell surface expression of TRAIL-R3/DcR1 and TRAIL-R4/DcR2 decoy receptors were very low. Conclusions： Sensitivity of gastric adenocarcinoma cells to TRAIL-induced apoptosis may be related to the expression of two death-induing receptors on the cell surface, especially DR4.

关 键 词：胃肿瘤 细胞凋亡 TRAIL TRAIL受体 

分 类 号：R735.2[医药卫生—肿瘤] R329.2[医药卫生—临床医学]