脑微血管内皮细胞条件培养液对星形胶质细胞活性的影响  

Effects of the Conditioned Medium of Normal Microvascular Endothelial Cells of Rat Brain on the Activity of Normal Astrocytes

作　　者：侯金才[1] 华茜[1] 潘彦舒[1] 陈会丛[1] 杜欢[1] 王筠[1] 李澎涛[1] 

机构地区：[1]北京中医药大学基础医学院

出　　处：《现代生物医学进展》2008年第5期814-816,共3页Progress in Modern Biomedicine

基　　金：国家"973"课题(2005CB523311);国家自然科学基金课题(30572284);国家"十一五"支撑计划(2006BAI08B05-04)资助

摘　　要：目的:观察脑微血管内皮细胞与星形胶质细胞的相互关系,探讨血脑屏障维持脑内环境稳定的生理学基础。方法:原代培养大鼠脑皮质微血管内皮细胞,传至三代,收集在指数生长期细胞生长48 h后的条件培养液;将条件培养液分别按20%、30%、40%、50%、60%、70%、80%、90%、100%不同浓度作用于星形胶质细胞,MTT法检测不同浓度内皮细胞条件培养液作用于星形胶质细胞24h、48 h后的活性变化。结果:48 h时间点的各浓度内皮细胞条件液组与相应的正常对照组相比差异均有显著统计学意义(P<0.01),内皮细胞条件液对星形胶质细胞表现出显著的抑制效应,而24 h的70%、80%、90%、100%浓度组与相应正常对照组相比也有显著统计学意义的差异(P<0.01),且有浓度依赖性。结论:正常脑微血管内皮细胞条件培养液抑制了正常星形胶质细胞的活性。HOU Jin-cai, HUA Qian, PAN Yan-shu, CHEN Hui-cong, DU Huan, WANG Jun, LI Peng-tao （1 School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China; 2 Anatomy Department, Qiqihar Medical University, Qiqihar 161042, China ） ABSTRACT Objective： To observe the interaction in microvascular endothelial cells and astrocytes, as well as to discuss the physiological foundation of brain homeostasis maintained by brain blood barrier. Methods： The primary cultured microvascular endothelial cells of rat brain were cultured until the 3rd generation and the conditioned medium was collected after 48 h culturing of exponential growing cells. The conditioned medium with final concentrations of 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% was added into astrocyte cultures respectively, and the effect of different concentrations of conditioned medium on the activities of astrocytes was determined through the measurement of MTT 24 h and 48 h after addition. Results： After 48 h, all concentrations of conditioned medium showed significant inhibition effect on the activity of astrocytes compared to normal control （ P〈0.01 ）, while after 24 h, 70%, 80%, 90% and 100% of conditioned medium showed inhibition as well compared to normal control （P〈0.01） and the inhibition effect was dose dependence. Conclusion： The conditioned medium of normal microvascular endothelial cells of rat brain could inhibit the activity of normal astrocytes.

关 键 词：脑微血管内皮细胞 血脑屏障 星形胶质细胞 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]