乙肝散对肝星状细胞增殖及凋亡的影响  

Effects of Yigansan on the proliferation and apoptosis of hepatic stellate cells

作　　者：程红球[1] 黄彩华[1] 邱杰文[1] 张赤志[2] 

机构地区：[1]汕头大学医学院第二附属医院,广东汕头515041 [2]湖北中医学院,湖北武汉430061

出　　处：《现代中西医结合杂志》2008年第15期2282-2283,2285,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：汕头市重点科技计划基金资助(2005116)

摘　　要：目的通过观察中药乙肝散的药物血清对肝星状细胞(HSC/T6)的增殖及凋亡的影响,从细胞学和分子生物学水平探讨中药乙肝散的抗纤维化的作用机制,为临床用药提供理论依据。方法①MMT法测定细胞增殖。②用流式细胞仪测定各组各浓度药物血清对HSC/T6作用后的DNA含量,低于G1期DNA含量主峰(亚G1期峰)的细胞为凋亡细胞。结果5%,10%和20%浓度的乙肝散的药物血清均对HSC/T6的增殖有显著的抑制作用,对HSC/T6的凋亡率分别为8.63%,14.56%和21.95%。结论中药乙肝散能抑制HSC/T6的增殖,诱导HSC/T6的凋亡,因此促进活化的HSC的凋亡可能是乙肝散抗肝纤维化的细胞分子学重要机制之一。Objective It is to observe the effect of Yigansan on the proliferation and the apoptosis of HSC/T6, in order to investigate its possible anti-fibrotic mechanism at cellular and molecular lever and provide an effective formula to clinical work. Methods ①MTT assay was used to observe the proliferation of HSC/T6. ②Flow cytometry was used to detect the contents of DNA in HSC/T6, The HSC/T6 with sub - G1 peaks in DNA histogram were apoptotic cells. Results The three concentrations as 5% , 10% and 20% of Yigansan＇s medicated sera could obviously inhibit the proliferation of HSC/T6 , the apoptosis rate were 8.63 %, 14.56 % and 21.95 %. Conclusion Yigansan can obviously inhibit the proliferation of HSC/T6, induce the apoptosis of HSC/T6. One of the important mechanisms of anti-fibrotic is inducing the apoptosis of activated HSC/T6.

关 键 词：乙肝散 肝星状细胞 细胞凋亡 

分 类 号：R051.262[医药卫生]