枯草芽孢杆菌B03液体发酵条件的优化  

Optimization of the Liquid Fermentation Condition of Bacillus subtilis B03

作　　者：赵达[1] 刘伟成[2] 裘季燕[2] 刘霆[2] 傅俊范[1] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110161 [2]北京市农林科学院植物保护环境保护研究所,北京100097

出　　处：《华北农学报》2008年第2期205-209,共5页Acta Agriculturae Boreali-Sinica

基　　金：北京市科技计划项目(No.D0706005000091,No.D0705002040291)

摘　　要：利用单因素筛选和均匀设计试验对拮抗性枯草芽孢杆菌B03发酵生产活菌体的液体培养基和发酵条件进行了研究,优化出了以经济、易得的玉米粉和豆饼粉为碳、氮源的最佳摇瓶发酵培养基,其配方为：玉米粉3.0%,豆饼粉6.0%,K2HPO4.3H2O 0.3%;最佳发酵条件为：种龄21-24 h,接种量5%,500 mL三角瓶装液量50 mL,初始pH值6.0,发酵温度35℃,摇床转速180 r/min,发酵周期60-66 h。利用优化后的培养基和发酵条件进行验证试验,获得了104.24×10^8-105.75×10^8cfu/mL的活菌体产量,较原始基础发酵培养基在常规培养条件下的菌体产量提高了96%以上。The medium and condition of liquid fermentation for biomass production of Bacillus subtilis B03 were optimized with mono-faetor experiment and uniform design. The most suitable medium for living cell production was formulated with the ingredients：3.0% corn meal,6.0% soybean oil meal and 0.3% K2HPO4·3H2O.The optimized fermentation process was as follows：the seed culture was cultured for 21 - 24 h,the pH of production medium was adjusted to 6.0 before sterilization, the fermentation was carried out in 500 mL erlenmeyer flask with 50 mL production medium inoculated with 5% seed culture and incubated at 35℃, 180 r/min for 60 - 66 h.The biomass density of 104.24 × 10^8 - 105.75 × 10^8 efu/mL was obtained with optimized formula, which was over 96% higher than that produced by basic medium and initial fermentation process.

关 键 词：枯草芽孢杆菌 菌体生产 液体发酵 均匀设计 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] S482.28[农业科学—农药学]