CD_4^+CD_(25)^+ T细胞变化与肺癌的关系  

Relationship between CD_4^+CD_(25)^+ T lymphocytes of peripheral blood and lung cancer

作　　者：杨宜娥[1] 付昕 林梅青[1] 宫奇林[1] 马万山[1] 曲仪庆[3] 

机构地区：[1]山东省千佛山医院,山东济南250014 [2]山东省电力中心医院 [3]山东大学齐鲁医院

出　　处：《山东医药》2008年第7期24-26,共3页Shandong Medical Journal

摘　　要：目的探讨外周血CD4+CD2+5T细胞及其所分泌的细胞因子与肺癌的临床相关性。方法选择68例肺癌患者和30例健康志愿者抽取静脉血,采用流式细胞术检测CD4+CD2+5T细胞水平和淋巴细胞亚群,ELISA方法测定TGFβ-、IL-10和IFN-γ的水平。结果肺癌患者外周血CD4+CD2+5T细胞水平明显高于正常对照组(P<0.05),且在肿瘤晚期(Ⅳ期)尤其明显。肺癌患者外周血中TGF-β、IL-10水平高于正常对照组(P<0.05),IFN-γ水平低于正常对照组(P<0.05)。结论肺癌患者外周血CD4+CD2+5T细胞水平及其抑制因子的升高与肿瘤免疫功能低下及肺癌的发生密切相关。Objective To evaluate the correlation between CD4^＋CD25^＋ regulatory T lyraphocytes and lung cancer. Methods Peripheral blood was obtained from 68 patients with lung cancer and 30 healthy persons. The percentages of CD4^＋CD25^＋ cells in total CD4^＋ cells, the expressive rates of CD4^＋, CD56^＋ and the ratio of CD4^＋/CD8^＋ were detected by flowe cytometry , the content of TGF-β,IL-10 and IFN-γ was detected by enzyme-labeled immunosorbent assay. Results The percent of CD4^＋CD25^＋ regulatory T lymphocytes in peripheral blood of lung cancer was higher than that of healthy volunteers （ P 〈0.05）. The level of TGF-β ,IL-10 in peripheral blood of lung cancer was higher than that of healthy volunteers（P 〈0. 05 ）. The level of IFN-γ in the lung cancer was lower than that of the healthy volunteers （ P 〈 0.05 ）. Conclusions The relative increase in CD4^＋CD25^＋ regulatory T cells may be related to immunosuppression and tumor progression in patients with lung cancer.

关 键 词：肺肿瘤 CD4^+CD25^+T细胞 转移生长因β 白细胞介素10 干扰素Γ 

分 类 号：R734.2[医药卫生—肿瘤]