白蔹多糖PAJM-Ⅰ的分离纯化和结构分析  

Isolation,purification and characterization of polysaccharide PAJM-Ⅰ from Ampelopsis japonica(Thunb)Makino

作　　者：林慧君[1] 陈卫[2] 

机构地区：[1]浙江省人民医院检验中心,杭州310014 [2]浙江大学生命科学学院,杭州310058

出　　处：《海峡药学》2008年第4期102-104,共3页Strait Pharmaceutical Journal

摘　　要：目的从白蔹中提取分离白蔹多糖,并研究其结构性质。方法经热水提取、乙醇沉淀、Sevag法脱蛋白后得到粗多糖PAJM,进一步用DEAE-Sapharose Fast Flow,Sephadex G-200分离纯化得到PAJM-Ⅰ,对分离到的主要多糖应用高碘酸氧化、高效凝胶渗透色谱(HPGPC),GC,GC-MS,IR等方法进行结构分析。结果提取纯化的白蔹多糖(PAJM-Ⅰ)是均一的多糖,相对分子量为3.25×104,鼠李糖,木糖,甘露糖和半乳糖组成摩尔比为:12.98∶23.52∶45.06∶18.44。分子中1→3,1→2,1→6连接的糖苷键数比为51%∶26%:23%。结论首次从白蔹中分离得到PAJM-Ⅰ均一多糖,且PAJM-Ⅰ含有α-D-葡萄吡喃糖。OBJECTIVE To isolate the water-soluble polysaccharides from Ampelopsis japonica （Thunb.） Makino and investigate its structural feature. METHODS The polysaccharides prepared from Ampelopsis japonica （Thunb.） Makino were purified with column chromatography on DEAE-Sapharose Fast Flow and Sephadex G-200. The structural feature of the main polysaccharide was elucidated by periodate oxidation , HPGPC , GC , GC-MS and IR respectively.RESULTS Two polysaccharides were obtained from Ampelopsis japonica （Thunb.） Makino. The results indicated that PAJM- Ⅰ was a glucomannan with a 12.98 ： 23.52 ： 45.06 ： 18.44 rhamnose ： xylose： mannose： galactose ratio and Mr was 3,25 × 10^4. Periodate oxidation analysis indicated PAJM- Ⅰ containing（1 →3 , 1→2, 1→6） glycosidic bond, appeared in the molar ratio of 51% ：26% ：23 % . CONCLUSION Two polysaccharides were isolated from this medicinal plant for the first time. PAJM- Ⅰ was a a-D-pyranyl glucomannan.

关 键 词：白蔹 多糖 分离 组成 

分 类 号：R914.3[医药卫生—药物化学]