牛初乳sIgA等电点的测定  

Detection of Isoelectric Point of Bovine Colostrum sIgA

作　　者：刘晓飞[1] 高学军[1] 李春[1] 姚永豪 周盛华[3] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨150030 [2]哈尔滨康普乳品有限公司 [3]东北农业大学动物医学学院

出　　处：《乳业科学与技术》2008年第3期115-116,共2页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金：黑龙江省科技厅(GA06B201-4)项目资助

摘　　要：采用等电聚焦电泳法测定牛初乳sIgA的等电点。牛初乳sIgA的pI为5.02-5.67,比相关报道更加精确。回归方程:y=0.7543x+3.1635,相关系数r=0.9989,表明线性关系良好,为牛初乳sIgA的离子交换层析制备条件的选择提供了可靠的理论依据。Isoelectric point of bovine colostrums slgA was detected by isoelectric focusing in this experiment, and an IEF method for the determination of protein was established. Isoelectric point of bovine colostrums sIgA is 5.02-5,67, this result is preciser than the related report, Regression equation：y =0.7543 x ＋3,1635, correlation coefficient： r=0,9989, this method had a good linear relationship. The experimental result offers a reliable theoretical basis for selection of ion exchange chromatography condition for preparation of bovine colostrums sIgA.

关 键 词：牛初乳 SIGA 等电点 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]