SS-GAG对高糖状态下血管内皮细胞NO释放及ET-1分泌的影响  

Effect of SS-GAG on ET1 and NO secretion of vascular endothelial cells damaged by highly concentrated glucose

作　　者：王瑞鑫[1] 王春波[2] 罗世滨[1] 李裕强[1] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209 [2]青岛大学医学院,山东青岛266071

出　　处：《山东大学学报（医学版）》2008年第4期357-361,共5页Journal of Shandong University:Health Sciences

基　　金：山东大学威海分校大学生科技创新项目(A07053)

摘　　要：目的观察高糖状态下扇贝裙边糖胺聚糖(scallop skirt-glycosaminogly-can,SS-GAG)对体外培养的血管内皮细胞(ECV-304)一氧化氮(NO)释放及内皮素-1(ET-1)分泌的影响。方法在体外培养生长良好的ECV-304分别加入不同浓度葡萄糖,建立高糖损伤细胞模型,选择葡萄糖终浓度为55.5 mmoL/L的培养液作为高糖环境,加入不同浓度SS-GAG继续培养至12、243、6、48 h,倒置显微镜下观察细胞生长状态,特异性硝酸还原酶法检测细胞内NO,放射免疫法测定ET-1。结果①高糖能抑制ECV-304细胞生长,细胞形态发生改变。SS-GAG减轻高糖对ECV-304损伤,SS-GAG保护组细胞形态基本完好;②高浓度葡萄糖促进ET-1的分泌,与正常对照比较差异有统计学意义(P<0.01)。而NO含量则随作用时间的延长表现出先升高后下降的动态变化过程,与正常对照比较差异有统计学意义(P<0.01)。SS-GAG对高糖状态下ECV-304细胞分泌ET-1、NO具有调节作用,使其趋于正常,与正常对照比较差异无统计学意义(P>0.05),与高糖比较差异有统计学意义(P<0.01)。结论高浓度葡萄糖对ECV-304具有明显的损伤作用,可直接导致ECV-304形态和功能异常,引起ET-1分泌增多以及损伤内皮NO系统使NO分泌失调。SS-GAG可减轻高糖对ECV-304细胞的损伤,通过调节ET和NO平衡,起到保护ECV-304作用。Objective To study the effects of scallop skirt glycosaminoglycan （SS-GAG） on ETI and NO secretion of ECV304 damaged by highly concentrated glucose. Methods The vascular endothehal cell ECV304 was cultured in vitro, and the damage model of endothelial cells was established by highly concentrated glucose. Different concentrations of SS-GAG were mixed with cell culture fluid containing highly concentrated glucose （55.5 mmol/L）, and cell morphology was observed after culturing for 12 h, 24 h, 36 h and 48 h. Nitric oxide （NO） inside cells was determined by the assay of nitric acid reductase. Radioimmunoassay was used to determine EY-1. Results Cell morphology was changed and proliferation was depressed under highly concentrated glucose, and a dose-dependent reaction occurred. SS-GAG reduced the damaging effects of highly concentrated glucose, and allowed these cells to keep their normal morphology. The concentration of ET-1 in the cell culture supematant was increased compared with the normal control group. Differences had statistics meaning compared with the normal control group （ P 〈 0.01）. The NO inside cells initially increased, and then reduced. Differences had statistical meaning compared with the normal control group （ P 〈 0.01）. There were obvious differences between the SS-GAG group and the negative control group. With modulation of SS-GAG, ET-1 and NO inclined to the normal level, which showed no difference with normal cells, but differences occurred between the KS-GAG and the highly concentrated glucose groups. Difference had statistics nmaning compared with the highly concentrated glucose group （ P 〈0.01） but not with the normal control group （ P 〉0.05）. Conclusions Highly concentrated glucose has obvious damaging effects on vascular endothelial cells, which may induce cell morphology change, dysfunction, ET1 increase and NO secretion. SS-GAG will reduce this damage and protect the cells by modulating the balance of ET1 and NO.

关 键 词：扇贝 糖胺聚糖 葡萄糖 血管内皮细胞 内皮素-1 一氧化氮 

分 类 号：R543[医药卫生—心血管疾病] R587.1[医药卫生—内科学]