基质金属蛋白酶-14和细胞外基质金属蛋白酶诱导因子在视网膜母细胞瘤中的表达及意义  

Expression of MMP-14 and EMMPRIN in retinoblastoma and their significance

作　　者：程育宏[1] 申家泉[1] 唐侠[1] 

机构地区：[1]山东大学附属省立医院眼科,济南250021

出　　处：《山东大学学报（医学版）》2008年第4期411-414,419,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Y2002C32)

摘　　要：目的探讨基质金属蛋白酶-14(matrix metalloproteinase-14,MMP-14)和细胞外基质金属蛋白酶诱导因子(extra-cellular matrix metalloproteinase inducer,EMMPRIN)在视网膜母细胞瘤(retinoblastoma,Rb)中的表达及其与临床病程的关系。方法用免疫组织化学SP法分别检测39例Rb中MMP-14及EMMPRIN的表达;用LEICA IM 50型全自动医学图像分析系统测量其平均灰度值,比较不同临床和病理阶段Rb中表达MMP-14和EMMPRIN的差异及二者阳性表达之间的关系。结果39例Rb中MMP-14阳性表达34例,占87.18%;EMMPRIN阳性表达33例,占84.62%;青光眼期Rb中MMP-14和EMMPRIN的表达明显高于眼内期(P<0.01及P<0.05),眼外期二者表达明显高于青光眼期(P<0.01及P<0.05);视神经浸润组Rb中MMP-14和EMMPRIN的表达明显高于无视神经浸润组(P<0.01及P<0.05);Rb中MMP-14和EMMPRIN的阳性表达水平呈密切相关(P<0.01)。结论MMP-14及EMMPRIN的表达水平可能与Rb的浸润转移有关,二者共同促进Rb的浸润转移。Objective To investigate the expressions of matrix metalloproteinase-14 （MMP-14） and extra-cellular matrix metalloproteinase inducer （EMMPRIN） in rednoblastoma（Rb） and the relationships between MMP-14 and EMMPRIN and tamor development. Methods Immunohistochemieal technique was used to determine the expressions of MMP-14 and EMMPRIN in 39 cases eft paraffin embedded Rb samples. Quantitative analysis of the expressions of MMP-14 and EMMPRIN were assessed by measuring the mean gray scale of Rb tissue with the LEICA IM 50 Color Pathologic Analysis System. Differences of the expreasions eft MMP-14 and EMMPRIN in each clinical and pathological stage were statistically analyzed, and the same step was also undertaken to study the relationship between Rb with MMP-14 positive expression and that with EMMPRIN positive expression, Results Positive expression rate of MMP-14 was 87.18% （34/39） and of EMMPRIN was 84.62%（33/39）. The expressions of MMP-14 and EMMPRIN were significantly higher in tamors of the glaucomatous stage than those in the intraoeular stage （ P 〈0.01 and P 〈 0.05）, and the same conclusion can be concluded between those in the extra-ocular stage and those in the glaucomatous stage （ P 〈0.01 and P 〈0.05）, also the expressions of MMP-14 and EMMPRIN were significantly higher in tumors with optic nerve invasion than those without optic nerve invasion （ P 〈0.01 and P 〈0.05）. There was a statistically significant positive correlation between the expression of MMP-14 and the expression of EMMPRIN （ P 〈 0. 01 ）. Conclusions Positive expression levels of MMP-14 and EMMPRIN may correlate with tumor invasion and metastasis and they may facilitate the Rb＇ invasion and metastasis together

关 键 词：视网膜母细胞瘤 基质金属蛋白酶-14 细胞外基质金属蛋白酶诱导因子 免疫组织化学 

分 类 号：R739.72[医药卫生—肿瘤]