蛋白激酶B siRNA转染对胃癌SGC-7901细胞生长增殖的影响  

In vitro growth inhibition of gastric cancer cells (MFC) by siRNA targeting PKB

作　　者：杨策尧[1] 郑斌[2] 段文晶[3] 段体德[3] 贾伟[1] 

机构地区：[1]昆明医学院第一临床学院医学研究中心,云南昆明650032 [2]云南省昆明市延安医院外科,云南昆明650051 [3]昆明医学院第一临床学院外科,云南昆明650032

出　　处：《中国普通外科杂志》2008年第4期346-349,共4页China Journal of General Surgery

摘　　要：目的探讨蛋白激酶B(PKB)基因沉默对人胃癌SGC-7901细胞增殖的作用。方法应用基因转染技术将蛋白激酶B家族的Akt2siRNA转染至胃癌细胞中,采用Western blot、琼脂糖凝胶电泳、流式细胞术MTT等方法观察和检测Akt2siRNA转染后对转染组细胞生长和增殖的影响。结果与对照组比较,转染Akt2siRNA组的胃癌SGC-7901细胞生长、增殖速度明显减缓(P<0.01),Akt2siRNA组较对照组细胞的Akt2蛋白表达水平显著下调(P<0.01);转染组出现梯状凋亡条带;细胞周期显示转染组细胞聚集在G2/M期。结论应用RNAi使蛋白激酶B基因沉默能使人胃癌SGC-7901细胞的生长受到抑制,胃癌细胞增殖速度减缓,其作用机理可能通过诱导细胞凋亡和抑制PI3/K信号传导通路而实现。Akt2有望成为胃癌基因治疗的新靶点。Objective To observe the effect of PKB gene silencing on the growth of gastric cancer cell line SGC-7901 in vitro. Methods Gene transfection technique was used to transfect AKt2 siRNA into gastric cancer cells. Akt2 expression was detected by RNAi technique, Akt2 protein level was detected by Western blot, and the change of cell cycle distribution and apoptosis of SGC-7901 cells were detected by flow-cytometry, SGC-7901 proliferation was measured by MTT method. Results After SGC-7901 cells transfected with Akt2 siRNA, the expression of protein level decreased obviously （ P 〈 0. 01 ） . Compared with control group, the transfected group had more SGC-7901 cells accumulated at G2/M phase, and proliferation rate was reduced （P 〈 0.01 ）. It also showed that the transfected group had apoptosis ladder, and the cell growth concentrated at GE/M phase. Conclusions siRNA targeting Akt2 could inhibit the proliferation of SGC-7901 cells in vitro. Induction of apoptosis and inhibition of PI3/K signaling pathway may be involved in its mechanism. Akt2 could be a novel target for gene therapy of gastric cancer.

关 键 词：胃肿瘤 RNA干扰 蛋白激酶B 细胞周期 细胞增殖 

分 类 号：R735.2[医药卫生—肿瘤]