巨噬细胞移动抑制因子对人结肠癌细胞增殖和血管形成的影响  

Effect of macrophage migration inhibitory factor on cell proliferation and angiogenesis in human colon carcinoma

作　　者：胡淞[1] 张才全[1] 倪春[1] 李建[1] 杨海平[1] 

机构地区：[1]重庆医科大学附属第一医院普通外科,重庆400016

出　　处：《中国普通外科杂志》2008年第4期350-354,共5页China Journal of General Surgery

摘　　要：目的探讨巨噬细胞移动抑制因子(MIF)对人结肠癌细胞增殖和血管形成的影响。方法检测不同浓度的抗MIF抗体(Anti-MIF)对人HCT-116结肠癌细胞株增殖及对血管内皮细胞生长因子(VEGF)的表达影响。结果100,200,400,800μg/LAnti-MIF处理HCT-116细胞48,72h后,细胞增殖明显受到抑制,且呈时间-剂量依赖性。100,200,400,800μg/LAnti-MIF处理HCT-116细胞72h后,流式细胞仪细胞周期分析表明G0/G1期细胞百分率上升,S期和G2/M期细胞百分率下降;与阴性对照组相比,Anti-MIF作用72h后VEGF蛋白表达明显减弱,并呈剂量依赖性(P<0.05)。结论MIF参与调节细胞的生长周期,并可能通过VEGF影响肿瘤血管形成,而在结肠癌的发病中发挥重要作用。Objective To study the effect of macrophage migration inhibitory factor （ MIF ） on cell proliferation and angiogenesis of human colon carcinoma. Methods Effect of different concentrations of Anti-human MIF antibody （Anti-MIF） on cell proliferative and expression of vascular endothelial growth factor （VEGF） of human colon carcinoma cell line HCT-116 cells was examined. Results After HCT-116 cells were exposed to Anti-MIF （100,200,400, and 800μg/L）for 48 and 72 h, HCT-116 cells growth was strongly inhibited in a dose and time-dependent manner; After exposure of the cells to Anti-MIF （100,200,400, and800μg/L） for 72 h, FCM analysis showed the ratio of G0/G1 phase cells increased, and the ration of S and G2 /M phase cells decreased. Comparison with the negative group, the expression of VEGF after the cells exposure to Anti-MIF for 72h were significantly decreased, and was in dose-dependent manner （P〈0. 05 ）. Conclusions MIF participates can modulat the cell growth cycle and can affect tumor neovascularization via VEGF. MIF plays an important role in the pathogenesis of colon carcinoma.

关 键 词：结肠肿瘤 巨噬细胞移动抑制因子 血管内皮生长因子 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]