实时荧光定量PCR检测HBV阳性孕妇胎盘组织TLR3的表达  

DETECTION OF TLR3 EXPRESSION IN PLACENTA OF PREGNANT WOMEN WITH HBV INFECTION BY QRTPCR

作　　者：车晓文[1] 王素萍[1] 李淑珍[1] 宋秀霞[1] 

机构地区：[1]山西医科大学公共卫生学院流行病学教研室,太原030001

出　　处：《现代预防医学》2008年第9期1686-1687,共2页Modern Preventive Medicine

基　　金：国家自然科学基金(30671794)

摘　　要：[目的]了解toll样受体3(TLR3)与孕妇乙肝病毒(HBV)感染的关系。[方法]提取组织总RNA,逆转录成mRNA,采用qRTPCR(real-timequantitative,RT-PCR实时荧光定量逆转录-多聚酶链反应相对定量的方法)分别检测正常孕妇和HBsAg阳性孕妇胎盘组织中TLR3mRNA,从基因水平比较TLR3在两组表达的差异。[结果]实时荧光定量PCR技术结果显示,病例组TLR3mRNA的含量低于对照组。,病例组Ct比值(TLR3/β-actin)为1.7029±0.20306,对照组为1.3882±0.19125,两组比较差异有统计学意义(t=-4.698,P=0.000﹤0.05)。[结论]胎盘组织中TLR3表达与孕妇HBV感染有一定关系。TLR3高表达可能是孕妇HBV感染的保护因素。[Objective]To explore the relationship of TLR3 in placenta and HBV infection of pregnant women. [Methods] Expressions of TLR3 mRNA in placentas of normal women and pregnant women with HBV infection were analyzed by real-time quantitative RT-PCR. Compared the different expressions in the two groups at the gene level. [ Results] The results from real-time fluorescence quantitative RT-PCR revealed that the content of TLR3 was lower in women with HBV infection than that in normal women. The difference had a statistical significance （t=-4.698, P = 0.000 〈 0.05）. [Conclusion] The expression of TLR3 has a association with HBV infection in pregnant women. High expression of TLR3 may effectively protect preg- nant women from HBV infection.

关 键 词：TLR3 HBV 胎盘 qRTPCR 

分 类 号：R152.6[医药卫生—营养与食品卫生学]