CD4^+CD25^+调节性T细胞在结直肠癌中的表达及其意义  

Expression of CD4^+CD25^+ regulatory T cells in colorectal cancer patients and its significance

作　　者：高素珍[1] 韩慧霞[1] 

机构地区：[1]南方医科大学病理学教研室,广东广州510515

出　　处：《中国癌症杂志》2008年第4期245-248,共4页China Oncology

摘　　要：背景与目的:CD4+CD25+调节性T细胞(Treg细胞)是体内自然发生的调节性T细胞的重要亚群,其在体内不仅参与自身免疫性疾病、移植排斥反应等,还在多种恶性肿瘤的发生、发展及免疫治疗中发挥重要作用。本研究探讨CD4+CD25+Treg细胞在结直肠癌中的表达及其意义。方法:收集30例结直肠癌患者的肿瘤组织及远离肿瘤部位组织,用流式细胞术和免疫组织化学方法对肿瘤组织中浸润的CD4+CD25+Treg细胞进行定量及定位分析。结果:CD4+CD25+Treg细胞含量:远离肿瘤部位组织为(5.5±1.3)%,肿瘤组织为(24.1±4.8)%,远离肿瘤部位组织与肿瘤组织相比,差异有显著性(t=5.155,P=0.002);有淋巴结转移者为(27.9±3.6)%,无淋巴结转移者为(20.3±1.3)%,差异有显著性(t=3.489,P=0.025)。结论:结直肠癌患者肿瘤组织中浸润的CD4+CD25+Treg细胞的比率显著升高,且与结直肠癌的进展有关。Background and purpose： CD4^＋CD25^＋ regulatory T （Treg） cells are an important naturally accruing subpopnlation of regulatory T cells. They not only prevent the spontaneous emergence of autoimmune diseases and contribute to the establishment of dominant tolerance on allogeneic transplantation, but also play an essential role in the generation, development and immunotherapy of many different kinds of malignant tumors. We studied the expression of CD4^＋CD25^＋ regulatory T cells（Tregs） in colorectal cancer patients and its significance. Methods： The tissues of tumors and counterpart normal tissue of 30 colorectal cancer patients were collected, the proportion of CD4^＋CD25^＋ Tregs as the percentage of the total CD4^＋ T cells were analyzed using flow cytometry and immunohistochemistry. Results： Compared with counterpart normal tissue（5.5±1.3） %, the percentage of CD4^＋CD25^＋ Tregs in tumor tissues was significantly higher （24. 1±4.8） % （t =5. 155, P =0.002）. The proportion of CD4^＋CD25^＋ Tregs in the patients with lymph nodes metastasis was （27.9±3.6） % which was significantly higher than that in patients without lymph nodes metastasis[ （20.3±1.3） % （ t = 3. 489, P = 0. 025） ]. Conclusions： The proportion of CD4^＋CD25^＋ Tregs in tumor tissues of colorectal cancer patients was significantly increased, and they may play an essential role in the development of colorectal cancer.

关 键 词：结直肠癌 T细胞 CD4^+CD25^+ 流式细胞术 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]