宫颈癌及上皮内瘤变中p16表达与预后关系的研究  

The Clinical Significance of Changes Expression of p16 in Cervical Carcinoma and Cervical Intraepithelial Neoplasm

作　　者：熊晓平[1] 杜丽英[1] 陈修己[1] 

机构地区：[1]解放军第94医院病理科,江西南昌330002

出　　处：《中华临床医学杂志》2008年第4期20-21,共2页Chinese Journal of Clinical Practical Medicine

摘　　要：目的研究宫颈癌及宫颈上皮内瘤变中p16基因表达的，临床意义。方法收集我院1996—2006年间妇科门诊活检及住院手术标本共164例，其中宫颈鳞状细胞癌86例，有淋巴结转移者22例，无淋巴结转移者28例，宫颈上皮内瘤变（CIN）78例，复习常规病理HE切片，并采用S-P免疫组化法对86例宫颈鳞癌和78例宫颈上皮内瘤变（CIN）蜡块标苓进行标记，观察P16基因的表达情况并进行对照分析。结果P16基因在宫颈鳞癌及CINⅠ、CINⅡ、CINⅢ中的阳性表达率分别为3g．3％（33／86）、70．0％（14／20）、484％（16／33）、44．0％（11／25），明显低于正常宫颈组织93.3％（28／30）（P〈0．05）；有淋巴结转移者和无淋巴结转移者P16基因阳性表达率分别为27．2％（6／22）、535％（15／28）（P〈005）。结论P16基因表达与宫颈鳞癌、CIN病变程度及淋巴结转移有关。Objective To study the clinical significance of changes of expression of p16 in cervical carcinoma and cervical intraepithelial neoplasia. Methods 164 cases of samples are from clinic and in-patient gynecologic department of our hospital（1996-2006）, inclnding 86 cases of cervical carcinoma（adenoid-ambulant, n=22; adenoid-unambulant,n=28） and 78 cases of cervical intraepithelial neoplasia（degree Ⅰ ,n=20; degree Ⅱ, n=33 ; degree Ⅲ, n=25）. The ratios ofpositive expression of the p16 in 164 cases of cervical carcinoma and cervical intraepithelial neoplasia were observed by S-P immune-histochemistry method. Results The levels of rations of positive expression of the p16 in the samples of cervical carcinoma and cervical intraepithclia neoplasia were lower than that of normal samples（P〈0.05）; The levels of ratios of positive expression of the p16 in the samples of cervical carcinoma with adenoid-ambulant were lower than that of the cervical carcinoma without adenoid-ambulant（P〈0.05）. Conclusion The expression of the p16 were related to the cervical carcinoma and its ambulant and the degree of cervical intraeplthelial neoplasia.

关 键 词：宫颈鳞状细胞癌 宫颈上皮内瘤变(CIN) 免疫纽化p16基因 

分 类 号：R-331[医药卫生] R737.23