银杏产黄酮内生真菌的分离与鉴定  被引量:15

Isolation and Identification of Endophytic Fungi of Falvonoid-Producing Ginkgo biloba

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作  者:韩晓丽[1] 康冀川[2] 何劲[1] 张禧庆[1] 黄庆华[1] 

机构地区:[1]贵州省农业生物工程重点实验室,贵阳550025 [2]贵州省生化工程中心,贵阳550025

出  处:《菌物研究》2008年第1期40-45,共6页Journal of Fungal Research

基  金:财政部产业技术成果转化项目(05071)

摘  要:为实现真菌发酵生产黄酮,用HPLC和氯化铝比色法,从四川、贵州、山东等地采集的银杏根、茎、叶中分离到7株产黄酮真菌。分子和形态学鉴定结果表明:这些菌株分别属于Colletotrichum和Fusarium,菌株QC102经鉴定为Colletotrichumacutatum Simmonds,其总黄酮产量为8.2μg/mL。Seven fungal isolates were found to produce falvonoids which were acquired from root, stem and leaves of Ginkgo biloba L. collected in the areas of Sichuan, Guizhou and Shandong provinces in Chi- na by means of HPLC and AlCl3 chromatometry. The further molecular and morphological identification indicated that these fungal isolates belonged to Colletotrichum and Fusarium respectively. The isolate QC102 was indentified as C. acutatum Simmonds which can produce falvonoids up to 8.2μg/mL.

关 键 词:银杏 内生真菌 黄酮 槲皮素 HPLC 

分 类 号:Q949.32[生物学—植物学] S792.95[农业科学—林木遗传育种]

 

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