心肌营养素-1基因转染对大鼠脑损伤的保护作用  

Protective effects of cardiotrophin-1 gene transfection mediated by adnovirus on traumatic brain injuries

作　　者：杨云华[1] 廖维宏[1] 李红运[1] 伍亚民[1] 张正丰[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所第三研究室,重庆400042

出　　处：《中华创伤杂志》2008年第5期355-359,共5页Chinese Journal of Trauma

摘　　要：目的在体观察腺病毒介导的心肌营养素-1基因（Adv—CTl）脑内转染对创伤大脑组织细胞的生物效应，探讨CT-1对创伤性脑损伤（TBI）治疗的作用和机制。方法以Allen方法建立大鼠TBI模型，利用Adv—CT1脑内转染技术，以Nissl染色、原位细胞凋亡检测、流式细胞凋亡检测等技术，观察CT-1基因治疗TBI后创伤大脑细胞凋亡及存活的变化及规律。结果TBI后12h-7d创伤对照组大脑皮层和海马等损伤脑区细胞凋亡明显增加，存活细胞减少；利用Adv—CT1脑内转染治疗TBI，伤后12h-14d损伤脑区凋亡细胞较创伤对照组减少，存活细胞增加。结论CT-1可减少TBI后脑细胞凋亡发生，增加脑细胞的存活数量，对损伤脑区细胞的生存有保护作用。Objective To observe biological effect of cardiotrophin-1 （Adv-CT1） gene transfection mediated by adnovirus on traumatic brain injuries （TBI） in-vivo and discuss the role and mechanism of Adv-CT1 on TBI. Methods A rat TBI model was established by Allen method. After Adv-CT1 was transferred into the injured brain by adnovirus, the effect of CT-1 on apoptosis and survival of neurons after TBI was determined by means of Nissl staining, TUNEL and flow cytometry apoptosis assay. Results Apoptotic cells were increased but the survived cells decreased in the injured cortical brain and hippocampus from 12 hours to 14 days after TBI in the control group. As compared with control group, Adv-CT1 treatment reversed this situation to some degrees. Conclusion CT-1 has neuroprotective effect on neurons after TBI by reducing apoptosis of neurons.

关 键 词：脑损伤 心肌营养素-1 基因治疗 凋亡 

分 类 号：R741[医药卫生—神经病学与精神病学]