α晶体蛋白对LPS刺激及视神经损伤后大鼠视网膜小胶质细胞增殖及数量的影响  

Inhibition of α-crystalnn on proliferation of rat retinal microglia following optic nerve injury

作　　者：吴楠[1] 王艳华[1] 王晓芹[1] 刘东宁[1] 应希[1] 张莉[1] 王一[1] 

机构地区：[1]第三军医大学附属西南医院眼科,重庆400038

出　　处：《中华创伤杂志》2008年第5期390-393,共4页Chinese Journal of Trauma

基　　金：国家重点基础研究发展规划资助项目（2007CB512203）;全军医学科研“十一五”计划资助项目（06G072）

摘　　要：目的研究α晶体蛋白对大鼠视网膜小胶质细胞增殖能力及视神经损伤后小胶质细胞数量的影响。方法脂多糖激活离体培养视网膜小胶质细胞，模拟视神经损伤，采用MTr分析α晶体蛋白对激活的小胶质细胞增殖能力的影响；建立大鼠视神经不全损伤模型，损伤后玻璃体腔注射α晶体蛋白，采用视网膜铺片及免疫荧光标记小胶质细胞并计数，比较不同组小胶质细胞的数量。结果10^-6g／L～10^-2g／L浓度的脂多糖均可激活小胶质细胞（P〈0．05），10^-6g／L和10^-4g／L浓度的α晶体蛋白可明显抑制10^-6g／L浓度的脂多糖激活的小胶质细胞的增殖；视神经损伤1～3周，α晶体蛋白注射组小胶质细胞的数量明显少于损伤组（P〈0．05）。结论α晶体蛋白可抑制视网膜上小胶质细胞的增殖和活化，减轻小胶质细胞对RGCs的过度吞噬和继发性损害，可能是视神经损伤后α晶体蛋白间接保护RGCs存活的另一机制。Objective To investigate the effect of α-crystallin on the proliferation of rat retinal microglia after optic nerve injury. Methods The effect of α-crystallin on number and proliferation of microglia were analyzed by MTT assay. After the rat model with optic nerve injury was established, α- crystallin was injected into vitreous cavity and the microglia cell number were counted and compared by retinal flat counting and immunofluorescence labeling in different groups. Results The proliferation and activation of microglia cells could be stimulated by LPS at 10^-6g/L to 10^-2g/L.α-crystallin at 10^-4g/L and 10^-6 g/L could inhibit proliferation and activation of microglia cells. Compared to BSA injection group, α-crystallin could inhibit more significantly the number of microglia cells 1-3 weeks after injury （P 〈 0.05）. Conclusions α-crystallin can inhibit proliferation and activation of retinal microglia and alleviate overphagocytosis and secondary damage of retinal microglia to retinal ganglion cells （ RGCs）, which may be another mechanism that α-crystallin contributes to indirect protection of RGCs.

关 键 词：创伤和损伤 视神经 小神经胶质细胞 视网膜 脂多糖类 晶体蛋白类 

分 类 号：R779.1[医药卫生—眼科]