硒在大蒜体内的生物富集及其抗氧化作用  

Studies on the Biological Accumulation and Antioxidation of Selenium in Garlic ( Allium sativum L. )

作　　者：段咏新[1,2] 傅庭治[1,2] 傅家瑞[1,2] 

机构地区：[1]中山大学生物科学与技术系 [2]南京大学生物科学与技术系

出　　处：《园艺学报》1997年第4期343-347,共5页Acta Horticulturae Sinica

基　　金：国家自然科学基金

摘　　要：大蒜对硒具有强的生物富集作用，并能将吸收的无机硒的７８．２４％转化为有机硒。经ＳｅｐｈａｄｅｘＧ－１５０柱层析，聚丙烯酰胺凝胶电泳，ＳＤＳ－聚丙烯酰胺凝胶电泳，首次从富硒大蒜中分离到一种分子量为３００００Ｄ的含硒蛋白，进一步研究其抗氧化作用，发现该蛋白能提高谷胱甘肽过氧化物酶、超氧化物歧化酶、过氧化氢酶活性，降低过氧化物酶活性。ＥＰＲ表明，Ｎａ２ＳｅＯ３处理组大蒜体内活性氧相对含量比对照低３１．９１％。The garlic possessed a great ability of Se biological accumulation. 78.24% inorganic Se absorbed was changed into organic Se. A kind of Se protein was isolated by column chromatography on Sephadex G-150, PAGE and SDS-PAGE. 30000D was determined. Its antioxidation was further researched. It increased the activities of glutathione peroxidase, superoxide dismutase,catalase and decreased the activity of peroxidase. EPR indicated that the active oxygen content was 31.91% lower in NaSeO 3 treatment than that in the control.

关 键 词：大蒜 硒 生物富集 硒蛋白 抗氧化作用 

分 类 号：S633.401[农业科学—蔬菜学]