p16基因在K562/A02细胞株表达情况的研究  

Research of expression of p16 gene in K562/A02 cell line

作　　者：寿倍明[1] 陈宝安[2] 周冬蕊[3] 刘德龙[4] 丁家华[2] 高冲[2] 孙耘玉[2] 王骏[2] 程坚[2] 赵刚[2] 宋慧慧[2] 鲍文[2] 陆祖宏[3] 

机构地区：[1]东南大学临床医学院,江苏南京210009 [2]东南大学附属中大医院血液科,江苏南京210009 [3]东南大学生物科学与医学工程学院,江苏南京210096 [4]纽约医学院医学部,美国纽约10595

出　　处：《东南大学学报（医学版）》2008年第3期151-153,共3页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(39970832)

摘　　要：目的:研究p16基因在K562/A02细胞株的表达情况及其与耐药的关系。方法:用PCR法对慢性髓系白血病细胞株K562及其耐药细胞株K562/A02进行p16基因缺失检测。结果:K562及K562/A02细胞株经PCR扩增后,均未扩出相应条带。结论:K562/A02细胞株与K562细胞株一样,均表现为p16基因缺失。Objective To discuss the expression of p16 gene in K562/A02 cell line and the relationship between the expression of p16 gene and muhidrug resistance. Method Polymerase chain reaction method was used to detect the expression of p16 gene. Result After PCR, both K562 and K562/A02 cell line had not been found to have amplified stripes. Conclusion Both K562 and K562/A02 cell line show deletion of p16 gene.

关 键 词：P16基因 K562细胞株 K562/A02细胞株 表达 缺失 

分 类 号：Q786[生物学—分子生物学] R733.72[医药卫生—肿瘤]