超声微泡辅助干细胞移植治疗急性心肌梗死的实验研究  

Experimental study of ultrasound-mediated microbubble destruction in myocardium-targeted delivery of bone marrow mesenchymal stem cells to treat myocardial infarction

作　　者：徐琢[1] 冯毅[1] 童嘉毅[1] 李金鹆[1] 马根山[1] 沈成兴[1] 祁春梅[1] 苏亚民[1] 张晓黎[1] 杨芳[2] 张宇[2] 

机构地区：[1]东南大学临床医学院,江苏南京210009 [2]东南大学生物科学与医学工程学院,江苏南京210096

出　　处：《东南大学学报（医学版）》2008年第3期192-194,F0003,共4页Journal of Southeast University(Medical Science Edition)

基　　金：东南大学博士科研启动基金资助项目(9290002296)

摘　　要：目的：探讨超声微泡在自体骨髓间充质干细胞（BMMSCs）移植治疗猪急性心肌梗死（AMI）改善心功能中的作用。方法：选取14只3～4月龄小型家猪，抽取骨髓，分离、培养、标记BMMSCs，球囊封堵猪左冠状动脉前降支（LAD）建立AMI模型，分为实验组（超声微泡作用下移植干细胞，n=8）和对照组（无超声微泡作用，n=6），7d后向LAD梗死处输注自体BMMSCs移植治疗。在移植前1d和移植后6周分别行64层螺旋CT心脏扫描及组织病理学检查。结果：与对照组相比。实验组心功能改善更为明显，梗死区毛细血管数（16．37±3．20 vs 59．50±1．87，P〈0．05）、普鲁士蓝染色阳性细胞数（71．63±14．09 vs 540．67±9．48，P〈0．05）、结蛋白免疫组化染色阳性细胞数（31．88±4．16 vs 519．00±2．28，P〈0．05）均明显增加。结论：超声微泡增效自体BMMSCs移植治疗AMI，能更有效地改善心肌梗死后的心功能，促进血管新生。Objective To study effects of ultrasound-mediated microbubble destruction in myocardium-targeted delivery of autologus bone marrow mesenchymal stem cells （BMMSCs） to treat acute myocardial infarction by improving heart function. Methods BMMSCs of 14 Chinese minipigs, aged 3 -4 months old, were selected, cultured and labeled by suerparamagnetic iron oxide. AMI model was made by blocking blood stream of left anterior descending branch. Pigs were devided into 2 groups ： experiment group （ BMMSCs ＋ Utrasound ＋ Bubble, n = 8 ） and control group （ BMMSCs, n = 6）. After 7 days, BMMSCs were transplanted into AMI areas of each host heart through intracoronary way. 64-slice multidetector computed tomography were performed to observe left ventricular function 1 day before and 6 weeks after transplantion. The histological analysis was conducted. Results Compared to the control group, the heart function was improved more significantinly in the experiment group. The capillary numbers of experiment group were more than those of the control group（ 16.37 ± 3.20 vs 9.50 ± 1.87, P 〈 0.05 ） ,the prussian blue-positive stained cells in experiment group outnumbered those in the control group（71.63 ± 14.09 vs 40.67 ± 9.48, P 〈 O. 05 ）, so did the desmin-positive cells （31.88 ± 4.16 vs 19. O0 ± 2.28, P 〈 O. 05 ）. Conclusion Ultrasound-mediated microbubble destruction shows favorable effects on myocardium delivery of autologus BMMSCs to treat acute myocardial infarction by improving heart function and angiogenesis.

关 键 词：超声 微泡 骨髓间充质干细胞 干细胞移植 急性心肌梗死 动物模型 猪 

分 类 号：R-33[医药卫生] R541.4