紫杉木霉ZJUF0986抗真菌活性代谢产物分离提取及其活性研究  

Separation and bioactive of antifungal metabolites of Trichoderma taxi ZJUF0986

作　　者：章初龙[1] 王国平[2] 郑必强[2] 陈绍瑗[1] 林福呈[1] 

机构地区：[1]浙江大学生物技术研究所,杭州310029 [2]建德市大洋化工有限公司,杭州311616

出　　处：《菌物学报》2008年第3期439-446,共8页Mycosystema

基　　金：浙江省重大科技攻关项目(No.2006C12008)

摘　　要：南方红豆杉Taxus chinensis var.mairei内生真菌紫杉木霉Trichoderma taxi菌株ZJUF0986发酵液对15种常见植物病原真菌具有很强的抑菌活性;用石油醚、乙酸乙酯和正丁醇对发酵液进行活性物质的提取分离,并通过硅胶柱层析和制备HPLC纯化,得到3个活性代谢产物Ⅰ、Ⅱ和Ⅲ,其中主要活性代谢产物Ⅰ具有广谱高效抑制植物病原真菌的特点,特别是对灰葡萄孢Botrytis cinerea、立枯丝核菌Rhizoctonia solani、核盘菌Sclerotinia sclerotiorum具有很强的抑菌活性,其IC50为1.1mg/L。FermentatiOn broth of endophytic fungus Trichoderma taxi ZJUF0986 has high antifungal activity to the common 15 species of phytopathogenic fungi. Three bioactive metabolites Ⅰ, Ⅱ and Ⅲ were obtained by extracted with petroleum ether, ethyl acetate and n-butanol and subsequent silica gel column chromatography and preparative HPLC. Among them; compound I, the main metabolit~, has strong broad-spectrum antifungal activity, especially to Botrytis cinerea, Rhizoctonia solani and Sclerotinia sclerotiorum with IC50 1.06, 1.08 and 1.13 mg/L, respectively.

关 键 词：内生真菌 发酵液 次生代谢产物 

分 类 号：S482.2[农业科学—农药学]