脆性组氨酸三联体和β-连接素在大肠癌中的表达及其意义  

Expression and Significance of FHIT,β-catenin in Colorectal Carcinoma

作　　者：张晓云[1] 张春雁[2] 孙曼萍[1] 王雨稼[1] 李棣华[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院病理科,上海200437 [2]上海中医药大学

出　　处：《中国临床医学》2008年第2期189-192,共4页Chinese Journal of Clinical Medicine

基　　金：上海中医药大学附属岳阳中西医结合医院院级科研项目

摘　　要：目的:探讨脆性组氨酸三联体(FHIT)和β-连接素(β-catenin)蛋白在大肠癌中的表达及其临床病理学意义。方法:采用免疫组织化学EnVision两步法,检测76例大肠癌及癌周非瘤粘膜组织、30例腺瘤标本的FHIT和β-catenin表达。结果:腺癌组织中FHIT表达降低或缺失率为51.3%,明显高于腺瘤组(6.7%)(P<0.01)。在癌的组织学分级中显示高分化组占10%,与中分化(56%)或低分化组(62.5%)相比差异显著(P<0.05)。大肠癌、大肠腺瘤组织中β-catenin的异常表达率分别为86.8%、73.3%,均显著高于癌旁非瘤黏膜组织(0%)(P<0.01)。在肿瘤的组织学分级中,β-catenin的异常表达率在中、低分化组为90.9%,显著高于高分化组(60%)(P<0.05);在临床分期中,DukesC及D期的β-catenin异常表达率为95.7%,显著高于DukesA及B期(72.4%)(P<0.01);伴淋巴结转移的β-catenin的异常表达率为95.3%,显著高于无淋巴结转移(75.8%)(P<0.05)。β-catenin异常表达与FHIT表达降低或缺失之间呈显著正相关关系(r=0.244,P<0.05)。结论:FHIT表达降低或缺失及其β-catenin的异常表达在大肠癌的发生发展中起重要作用。部分大肠癌组织中β-catenin的异常表达可能由抑癌基因FHIT的表达降低或缺失所致。Objective：To investigate the expression of fragile histidine triad （FHIT） and β-catenin protein and their correlations with clinicopathologic features in colorectal carcinomas. Methods：The expression of FHIT and β-catenin protein was investigated in 76 primary colorectal carcinomas, adjacent noncancerous mucosa and 30 adenomas using EnVision immunohistochemistry. Results.. The reduction or loss rate of FHIT expression was 51.3 % in colorectal carcinomas, and significantly higher than that in adenomas （6.70%） （P〈0.01）： 10% well-differentiated, 56%moderately-differentiated, 62.5%poorly-differentiated carcinomas, the frequency being significantly higher in the latter two than that in the former （P〈0.05）. The abnormal expression rate of β-catenin was 86.8%, 73.3 % in colorectal carcinomas and adenomas respectively. They were significantly higher than that in adjacent noncancerous mucosa（0~） （P%0.01）. The abnormal expression rate of β-catenin was significantly higher in moderately and poorly-differentiated carcinomas（90.9% ） than that in well-differentiated carcinomas（60% ） （P〈0. 05）. It was also significantly higher in late stage carcinomas（Dukes stage C ＋ D） （95.7%） （P〈0.01） and carcinomas with lymph node metastasis（95.3%） （P〈0.05）. The abnormal expression of β-catenin was in positive correlation with the reduction or loss expression of FHIT（r = 0. 244, P〈0.05）. Conclusion . The abnormal expression of β-catenin and the reduction or loss expression of FHIT play important roles in the carcinogenesis and progression of colorectal carcinoma. The abnormal expression of β-catenin may be induced by the reduction or loss expression of FHIT in some colorectal carcinomas.

关 键 词：大肠癌 脆性组氨酸三联体 Β-连接素 临床病理学 

分 类 号：R735.3[医药卫生—肿瘤]