志贺菌临床分离株耐多药与调控基因突变关系  

Aanlysis on acrR、marOR multi-drug regulated gene mutations in clinical isolates of Shigella

作　　者：吕锐利[1] 段广才[1] 宋春花[1] 郗园林[1] 

机构地区：[1]郑州大学公共卫生学院流行病学教研室,郑州450001

出　　处：《中国公共卫生》2008年第5期568-570,共3页Chinese Journal of Public Health

基　　金：河南省科技公关项目资助课题(0424410035)

摘　　要：目的研究志贺菌的耐多药性及其耐多药调控基因acrR、marOR的变异性。方法对56株临床分离的志贺菌进行四环素、氯霉素、氨苄青霉素、环丙沙星、诺氟沙星等5种药物的药敏试验,对其acrR、marOR基因进行聚合酶链反应(PCR)扩增后,TaqI限制性内切酶酶切与单链构象多态性(SSCP)分析。结果筛选出35株耐多药株与11株敏感野生株。56株临床分离的志贺菌属细菌的耐多药率为62.5%;35株耐多药株与11株敏感野生株均扩增出acrR、marOR基因,未发现基因缺失株;单链构象多态性分析发现,acrR基因突变率为6.52%,marOR基因突变率为10.87%,11株敏感野生株均未检出以上2种基因突变。结论志贺菌耐多药率较高,acrR、marOR基因突变可能是其耐多药的调控机制之一。Objective To study the multi - drug resistance and its regulation mechanism of Shigellae spp. Methods Fifty - six clinical isolates of Shigella were collected. Susceptibility tests of tetracycline （TE）, chloramphenicol （C）, ampicillin （AM）, ciprofloxacin（CIP）, and norfloxacin（NOR） were performed in all isolates. AcrR and marOR genes were amplified by polymerase chain reaction（PCR） respectively. The PCR products were digested by restriction endonuclease TaqI, and then analyzed by single strand conformation polymorphism（SSCP）. Results Thirty - five multi - drug strains and eleven wild type strains were selected. The rate of multi - drug resistance was 62.5 %. AcrR and ,mrOR genes were found in all strains and the lack of the two genes were not found. SSCP analysis found three multi - drug resistant strains（6.52 % ） carrying mutations in acrR gene and five multi- drug resistant strains（10.87% ） carrying mutations in marOR gene. No mutation was found in three wild type strains. Conclusion The Shigella spp. had high multi - drug resistance rate. The acrR and marOR gene mutations may play an important role in the regulation of multi - drug resistance in Shigella spp.

关 键 词：志贺菌 耐多药 acrR marOR 基因突变 

分 类 号：R378.2[医药卫生—病原生物学]