神经胶质瘤细胞对血管新生作用及其血管生成素-2表达的影响  

Facilitating the neogenesis of blood vessels of gilomas cells and its effection on expression of angiogenin-2

作　　者：李宝月[1] 刘政操[2] 姚琦[1] 顾苏宁[1] 

机构地区：[1]沈阳市第七人民医院病理科,辽宁沈阳110003 [2]中国医科大学附属盛京医院病理科,辽宁沈阳110003

出　　处：《中国医药导报》2008年第13期14-16,共3页China Medical Herald

摘　　要：目的:探讨人神经胶质瘤细胞对血管新生的影响及作用机制。方法:体外分离培养人神经胶质瘤细胞,用免疫细胞化学法检测不同级别的肿瘤细胞中血管生成素-2的表达,采用图像分析软件进行光密度(IOD)的测量;培养人脐带静脉内皮细胞并应用Matrigel建立血管形成的体外培养体系,检测肿瘤细胞对血管新生的影响。结果:血管生成素-2在神经胶质瘤细胞中的表达强度与瘤组织的分级呈正相关(P<0.05);肿瘤细胞诱导肿瘤血管形成呈浓度依赖效应(P<0.05)。结论:神经胶质瘤细胞可以诱导肿瘤血管新生,血管生成素-2在神经胶质瘤细胞中的表达增强与肿瘤血管新生过程相关。Objective： To discuss the influence of the role of human neurospongioma cells infaeilitating human vascular endothelial cells and expression of neurospongioma angiogenin-2.Methods：HUVECs were euhured by modified Jaffe methods and induced to develop tubular structure by Matrigel.Thus set up a stable angiogenesis system and added the stimulating factor.Neurospongioma cells were fractional euhivated in vitro and the expression of Ang-2 protein was detected by immuneytochemieal methods. Finally the dale was analyzed by statisties.Results：HUVECs could be induced to form tubular structure by Matrigel in vitro. After treatment with the conditional medium of tumor cells, the number of tubules increased in a dose-dependent manner. The expression of Ang-2 increased in neurospongioma cells.Conclusion：Anglogenesis can be induced by neurospongioma cells;the expression of Ang-2 in neurospongioma upregulates and in positive correlation with grade of tumor.

关 键 词：肿瘤 血管新生 血管内皮生长因子 血管生成素 

分 类 号：R364.3[医药卫生—病理学]