亚硝酸钠对地鼠生殖毒性的研究  

The study about the reproductive toxicity reaction of sodium nitrite on the hamster.

作　　者：顾宪敏[1] 马保华[1] 禹卉[2] 董平[3] 

机构地区：[1]山东大学医学院组织学与胚胎学教研室,济南250012 [2]山东大学医学院形态学实验室,济南250012 [3]南京大学医学院解剖学与组胚教研室

出　　处：《现代妇产科进展》2008年第4期289-293,共5页Progress in Obstetrics and Gynecology

基　　金：山东省自然科学基金资助课题(No:Y2004C08)

摘　　要：目的:探讨亚硝酸钠的生殖毒性。方法:(1)体内实验:随机将未经产雌性成熟金黄地鼠160只分为对照组和实验组,实验组分别腹腔注射不同浓度的亚硝酸钠[2、4、8mg/(kg·d)],对照组给予生理盐水。于光镜及电镜下观察卵巢颗粒细胞的形态改变;(2)体外实验:细胞培养并对培养的卵巢颗粒细胞进行钙通道蛋白免疫印迹半定量实验以观察钙通道蛋白的表达量。结果:(1)予以亚硝酸钠4mg/(kg·d)和8mg/(kg·d)负荷5天后,地鼠卵巢有毛细血管扩张充血、卵巢颗粒细胞排列紊乱等明显变化;(2)予以亚硝酸钠8mg/(kg·d)负荷5天后,地鼠有卵泡透明带变窄、其内(颗粒细胞)微绒毛减少、颗粒细胞内脂滴和滑面内质网也减少等明显变化;(3)体外培养系统内追加不同负荷剂量的亚硝酸钠24h后,培养的颗粒细胞相应的钙通道蛋白表达呈剂量依赖性下降(P<0.05 vs对照组);(4)腹腔内注射不同负荷剂量的亚硝酸钠5天后,相应的地鼠合笼交配后生仔率也呈现剂量依赖性下降(P<0.05 vs对照组)。结论:亚硝酸钠影响地鼠卵巢颗粒细胞正常结构的发育,干扰地鼠生育能力。Objective：To investigate the reproductive toxicity of sodium nitrite. Methods ： （ 1 ） Experiment in vivo ： 160 nulliparity female hamsters were randomly divided into experimental groups and control groups. The experimental groups were given sodium nitrite of different concentrations[2,4,8 mg/（ kg· d） ] by intraperitoneal injection respectively,and the same volume of normal saline was given in the control group. The changes of granulose cell morphology were observed by light microscopy and electron microscopy. （2）Experiment in vitro： the expression of calciphorin was determined by immunoblotting semi-quantitative analysis. Results： （1） Five days after given sodium nitrite of different concentrations 4mg/（ kg · d） and 8mg/ （kg· d）, the irregular ovarian follicle and dilatation of blood vessel could be seen in the ovaties of hamster. （2） Five days after given sodium nitrite of 8mg/（ kg· d） concentration,the pellucida of ovarian follicle became narrow, the microvillus and lipid droplets and the smooth endoplasmic reticulum of granulose cell were reduced definitely. （3） 24 hours after given different concentrations of sodium nitrite, the expressions of the calcium channel proteins of the cultured ovarian granulose cells decreased in a dose-dependent manner （ P 〈 0.05 ）. （4） Five days after given rarious dosage of sodium injection, the birth rate was reduced in a dose-dependent manner. Conclusions：Sodium nitrite can significantly effect the normal structure of the ovarian and interfere fertility.

关 键 词：亚硝酸钠 钙通道蛋白 卵巢颗粒细胞 生育能力 地鼠 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]