光动力疗法对胰腺癌移植瘤的疗效及其时-效关系  

The anti-tumor effect of photodynamic therapy on transplanted pancreatic cancer model and its time-effect relationship

作　　者：贾林[1] 刘艳虹[1] 

机构地区：[1]广州医学院附属市一人民医院消化内科,广东510180

出　　处：《中华胰腺病杂志》2008年第2期95-97,共3页Chinese Journal of Pancreatology

基　　金：广东医学科学技术研究基金（A2004526）;2006年广东省社会发展领域科技计划项目（63082）

摘　　要：目的探讨光动力疗法（PDT）对人胰腺癌裸鼠移植瘤的疗效及其时-效关系。方法将人胰腺癌细胞SW1990皮下种植后形成的瘤块剪成1 mm^3组织块再移植至36只裸鼠皮下，待皮下移植瘤直径达0．8～1cm时按随机分组法分为3组：荷瘤对照组、光敏剂组（腹腔内注射Photosan 2 mg／kg体重）、光动力组（腹腔内注射Photosan 2 mg／kg体重，48h后予激光照射），每组12只。每周2次测量肿瘤大小，3周后取瘤称瘤重，计算瘤体积及抑瘤率。结果PDT组治疗后第6天瘤体积为（0．24±0．15）cm^3，显著小于荷瘤对照组的（0．43±0．18）cm^3和光敏剂组的（0．39±0．15）cm^3（P〈0．05），并随着时间延长，差异更加显著。但治疗后15d起，PDT组移植瘤体积又开始增大。治疗21d后，PDT组瘤重为（0．69±0．23）g，显著小于荷瘤对照组的（1．65±0．21）g和光敏剂组的（1．62±0．12）g（P〈0．05）。PDT组抑瘤率为58．18％，显著高于光敏剂组的1．80％（P〈0．05）。结论PDT对胰腺癌具有显著的抑制作用，起效快，但单用疗效维持时间不长。Objective To investigate the anti-tumor effects of photodynamic therapy （PDT） on human pancreatic cancer xenograft in nude mice and the time-effect relationship of PDT. Methods The animal model of human pancreatic cancer was developed by suturing small pieces of SW1990 tumors into the dorsum of nude mice. When the size of the tumors increased to 0.8 - 1.0 cm, 36 mice were randomly divided into three groups ： control group（ no treatment）, photosensitizer group （ Photosan 2mg/kg, abdominal cavity injection ）, photodynamic group （Photosan 2 mg/kg injection ＋ laser irradiation）. Each group included 12 mice. The tumor sizes were measured twice per week and the weights and volumes of the tumors were measured, and the tumor inhibitory rate was calculated three weeks later. Results The tumor volume of photodynamic group was （0.22 ± 0.12） mm^3 at the 6th day, which was significantly smaller than （0.43 ± 0.18） mm^3 of control group and （0.39 ± 0.15 ） mm^3 of photosensitizer group （P 〈 0.05）. 15 days later, the tumor volumes of PDT groups increased. 21 days later, the weight of the tumors in photodynamic group was （0.69±0.23） g, which was significantly lower than （ 1.65±0.21 ） g of control group and （ 1.62±0.12） g of photosensitizer group （P 〈 0.05 ）. The tumor inhibitory rate in photodynamic group was 58.18%, which was significantly higher than that of photosensitizer group （ 1.8%, P 〈 0.05 ）. Conclusions Photodynamic therapy had significant anti-tumor effect on human pancreatic cancer with quick-acting efficacy, but photodynamic therapy alone exerted efficacy only in the short term.

关 键 词：胰腺肿瘤 光动力疗法 时-效关系 

分 类 号：R735.9[医药卫生—肿瘤]