海藻糖对医用诊断工具酶活性保护研究  

STUDY ON STABILITY OF DIAGONOSTIC TOOL ENZYMES UNDER TREHALOSE PROTECTION

作　　者：黄成垠[1] 安国瑞[1] 王庆敏[1] 戴秀玉[2] 周坚[2] 

机构地区：[1]河北省沧州市中心医院检验科,沧州061001 [2]中国科学院微生物研究所,北京100080

出　　处：《微生物学通报》1997年第6期341-343,共3页Microbiology China

基　　金：河北省科委资助

摘　　要：研究了干燥条件下海藻糖对胆固醇氧化酶，胆固醇脂酶和过氧化物酶的活性保护作用，并对影响酶活性保存的其它因素进行了试验。加海藻糖干燥的以上三种诊断酶分别在70，55和37℃温度下放置40d，150d和210d后再次测定酶活，结果表明这三种酶的活性均保持在90％以上，而未加海藻糖保护的对照，活性降至20％以下。除海藻糖浓度外，缓冲系统中的介质、离于强度、pH是影响酶活的关键因素。The heat stability of three tool enzymes dried in trehalose at room temperature wasstudied for the possibility of preperation extraodinary stable enzyme reagent kit for serumcholesterol analysis. The cholesterol oxidase, cholesterol esterase and peroxidase have been driedin trehalose containing buffer and stored at 37c for 21O days, 55c for 15O days, 7Oc for4O days respectively. All of three enzymes achvity recovered more than 9O%. The trehaloseprotection is remarkably. In addition to concentration of trehalose, the buffer, ionic strength andpH are the main influence factors on stability of enzymes dried in trehalose.

关 键 词：海藻糖 诊断工具酶 实验室诊断 酶试剂 

分 类 号：R446[医药卫生—诊断学]