槲皮素联合顺铂对肺腺癌小鼠移植瘤Bcl-2和Bax表达的调控及意义  

The regulatory effects of quercetin combined with DDP on Bcl-2 and Bax expressions in transplanted lung adenocarcinoma in mice and their significance

作　　者：贺兼斌[1] 张贻秋[1] 向志[1] 唐建新[1] 易高众[1] 张平[2] 

机构地区：[1]湖南省怀化市第一人民医院呼吸内科,怀化418000 [2]南华大学附属第一医院呼吸内科,衡阳421001

出　　处：《肿瘤》2008年第5期386-389,共4页Tumor

基　　金：湖南省卫生厅科研基金资助项目(编号C2005033)

摘　　要：目的:观察槲皮素联合顺铂对肺腺癌LA795细胞的T739小鼠移植瘤的抑制作用,检测移植瘤中Bcl-2和Bax的表达水平及肿瘤细胞凋亡指数,探讨其作用机制。方法:将32只接种了LA795肺腺癌细胞的T739小鼠随机分成对照组(A组)、顺铂组(B组)、槲皮素组(C组)、槲皮素+顺铂组(D组)。用药16 d后,观察肿瘤生长情况,接种后24 d活杀各组小鼠,移植瘤标本称瘤体质量和测量体积,免疫组织化学和图像分析系统定量检测肿瘤组织的Bcl-2和Bax表达,原位凋亡TUNEL法检测肿瘤细胞凋亡指数。结果:B、C、D各用药组肿瘤的生长受到明显抑制,瘤体质量明显低于A组,其抑瘤率分别为38.57%、26.03%和51.58%,联合用药组抗瘤作用进一步增强。Bcl-2表达在A组高于B、C、D组,Bax表达在A组明显低于B、C、D组,各用药组与对照组相比差异均有统计学意义(P<0.05或P<0.01);肿瘤细胞的凋亡指数在各用药组都比对照组明显提高,差异有统计学意义(P<0.01)。结论:槲皮素联合顺铂可明显抑制肺腺癌细胞在小鼠体内的生长,其作用机制可能是通过调控肿瘤中Bcl-2和Bax的表达,从而抑制细胞增殖和促进细胞凋亡。Objective：To observe the inhibitory effects of quercetin combined with DDP on the growth of transplanted lung adenocarcinoma LA795 cells in 1739 mice and detect the expression levels of Bcl-2 and Bax and apoptotic index to explore the action mechanism for the combination therapy. Methods：Thirty-two T739 mice inoculated with LA795 lung adenocarcinoma cells were randomly divided into four groups with 8 mice in each group： control group （ A）, DDP treatment group （ B）, quercetin treatment group （ C）, DDP plus quercetin treatment group （D）. The tumor growth was observed on day 16 after drug administration. All mice were sacrificed on day 24 after inoculation. The weight and volume of transplanted tumors were recorded. The expression levels of Bcl-2 and Bax were quantified using immunohistochemical method and image analyzing system. The apoptotic index of transplanted tumors was measured by TdT-mediated dUTP-biotin nick end labeling（TUNEL） method. Results：The growth of transplanted tumors in groups B, C, and D were significantly inhibited. The weight of tumor body in the three groups were markedly lower than that of group A. The tumor inhibitory rates were 38.57%, 26.03%, and 51.58% in groups B, C, and D, respectively. The inhibitory effect of quercetin combined with DDP on tumor growth was enhanced compared with quercetin or DDP alone treatment. The expression of Bcl-2 was significantly higher but Bax was significantly lower in group A compared with group B, C, and D. The difference between the drug therapy groups and control group was significant （ P 〈 0. 05 or P 〈 0. 01 ）. The apoptotic index was significantly increased in the three drug therapy groups compared with control group and the difference was significant （ P 〈 0. 01 ）. Conclusion：The combination therapy for quercetin plus DDP significantly suppresses the growth of lung adenocarcinoma cells in mice. The action mechanism may be due to regulation of Bcl-2 and Bax expressions, thereby, inhibiting cell proliferation a

关 键 词：肺肿瘤 槲皮素 顺铂 原癌基因蛋白质C-BCL-2 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]