HPLC法测定复方氨酚苯海拉明片中对乙酰氨基酚、咖啡因、盐酸麻黄碱和盐酸苯海拉明的含量  

Simultaneous determination of paracetamol,caffeine,ephedrine hydrochloride and diphenhydramine hydro-chloride in compound paracetamol and diphenhydra-mine hydrochloride tablets by HPLC

作　　者：高广慧[1] 王晓黎[1] 董宇[1] 战祥友[1] 慕善学[2] 

机构地区：[1]辽宁省食品药品检验所,辽宁沈阳110023 [2]沈阳药科大学高职技术学院,辽宁沈阳110026

出　　处：《沈阳药科大学学报》2008年第5期384-387,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立同一色谱条件测定复方氨酚苯海拉明片中的对乙酰氨基酚、咖啡因、盐酸麻黄碱和盐酸苯海拉明含量的HPLC法。方法采用苯基柱(4.6 mm×250 mm,5μm),流动相为甲醇-pH 3.5的10 mmol.L-1乙酸铵溶液(体积比85∶15),检测波长为225 nm。结果对乙酰氨基酚、咖啡因、盐酸麻黄碱和盐酸苯海拉明的分离度好,对乙酰氨基酚、咖啡因、盐酸麻黄碱和盐酸苯海拉明的回收率分别为100.1%、100.0%、100.2%和100.6%(n=9)。结论本方法操作简单,结果准确,可以有效地控制复方氨酚苯海拉明片的质量。Objective To establish a method for determining the compound paracetamol and diphenhydramine hydrochloride tablets by HPLC. Methods Phenyl column was used and methanol-10 mmol·L^-1 pH3.5 ammonium acetate buffer（ V： V = 85： 15） was selected as mobile phase; the wavelength of detector was set at 225 nm. Results Paracetamol, caffeine, ephedrine hydrochloride and diphenhydramine hydrochloride were well separated. The average recoveries were 100.1%, 100.0%, 100.2% and 100.6% （ n = 9）, respectively. Conclusions This method is simple, accurate and convenient. It is very suitable for the quality control of the compound paracetamol and diphenhydramine hydrochloride tablets.

关 键 词：高效液相色谱法 复方氨酚苯海拉明片 对乙酰氨基酚 咖啡因 盐酸麻黄碱 盐酸苯海拉明 

分 类 号：R917[医药卫生—药物分析学]