特定有益菌对奶牛粪便中温厌氧消化体系稳定性和指示菌存活的影响  

The Effects of Selected Effective Bacteria on Gas Production and Survival of Indicative Bacteria During Mesophilic Anaerobic Digestion of Dairy Cattle Dung

作　　者：孙翠平[1] 柴同杰[1] 张红双[1] 姚美玲[1] 李晓霞[1] 段会勇[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《农业环境科学学报》2008年第3期1183-1189,共7页Journal of Agro-Environment Science

基　　金："十一五"国家科技支撑计划课题:"畜禽养殖污染物减排和废弃物资源循环利用技术研究与示范"之课题9"畜禽养殖环境污染物监测与减排技术研究"(2006BAD14B09)

摘　　要：本文研究了添加假单胞菌、芽孢杆菌、乳酸菌、酵母菌和放线菌对奶牛粪便中温(35℃)厌氧消化过程中消化体系稳定性和指示菌(粪大肠菌群、产气荚膜梭菌、厌氧菌和需氧菌)存活情况的影响。通过采用1L抽滤瓶作为消化罐,置于恒温箱内35℃模拟中温厌氧消化。检测厌氧消化过程中粪大肠菌群、产气荚膜梭菌、厌氧菌和需氧菌数量的变化,同时对挥发性脂肪酸(VFA)含量、NH4+-N含量和pH值等指标进行测定。结果发现,在厌氧消化体系中添加假单胞菌、芽孢杆菌、乳酸菌、酵母菌和放线菌对整个消化过程中VFA含量、NH4+-N含量和pH值没有显著影响(P>0.05),但可提高酸化阶段丁酸和乙酸的产量,降低丙酸的产量,有利于形成一个良好、稳定的厌氧消化体系。添加的微生物可以显著促进粪大肠菌群数量的减少(P<0.05),但对产气荚膜梭菌、厌氧菌和需氧菌的数量并没有显著的影响(P>0.05)。试验结果表明,假单胞菌、芽孢杆菌、乳酸菌、酵母菌和放线菌在厌氧消化处理畜禽排泄物过程中的应用有一定的理论推广价值,其实际应用的研究有待进一步开展。The study was to evaluate inactivation of indicative bacteria or bacterial flora： Fecal Coliforms, C. perfringens （Clostridium pe（fringens）, the total number of anaerobic and aerobic bacteria, by bioaugmenting anaerobic digestion of cow dung slurry with a commercial product containing selected strains of bacteria from genera Lactobacillus, Pseudomonas, Microzymes and A ctinomycetes, along with ancillary organic compounds containing various micronutrients. Specifically, the effects of the bioaugment on the concentration of volatile fatty acids, NH4^＋ -N, the value of pH, the number of Fecal Collforms and C. pe（fringens during anaerobic digestion of dairy cattle dung slurry were studied. The results indicated that the bioaugmenting anaerobic digestion could reduce the number of Fecal Coliforrns significantly （P〈0.05）, as well as could be of advantage to form a better and more stable digestive system, however, no obvious effect on reduction of the number of C. pe （fringe ns, the total number of anaerobic and aerobic bacteria were observed. The bioaugmenting anaerobic digestion could not significantly affect the output of volatile fatty acids （VFA）, NH4^＋ -N and the value of pH （P〉0.05）. In brief, the bioaugmenting anaerobic digestion could improve anaerobic digestive microecology and could be used for treatment of dairy cattle dung slurry before their land application.

关 键 词：牛粪 中温厌氧消化 有益微生物 指示菌 

分 类 号：X172[环境科学与工程—环境科学]