啮齿类螺杆菌不同检测方法的比较  

Comparison of Different Diagnostic Methods for Detection of Rodent Helicobacter

作　　者：张丽芳[1] 刘星[1] 李红[1] 

机构地区：[1]中国医学科学院中国协和医科大学实验动物研究所,北京100021

出　　处：《中国比较医学杂志》2008年第5期62-65,F0003,共5页Chinese Journal of Comparative Medicine

基　　金：国家科技基础条件平台工作重点项目(编号:2003DIA7J033)

摘　　要：目的确定用于实验啮齿类动物常规螺杆菌检测方法。方法选用针对螺杆菌属16S rRNA属特异性的4对引物进行了引物选择、取材部位以及与分离培养法比较。结果引物P7/P8的敏感性优于其它3对,检出限可达0.01 pg,对阳性动物群检出率80%-100%;分离培养法检出率40%-50%;盲肠、结肠检出率无显著差异。结论以引物P7/P8的PCR方法作为啮齿类实验动物螺杆菌初筛方法,分离培养法作为验证方法,取材部位可在盲肠或结肠。Objective To confirm the routine diagnostic methods for detecting Helicobacter spp. on rodent laboratory animals. Methods Four pairs of 16S rRNA primers specific for Helicobacter genus were selected and compared with each other. Comparision of different sampling sites were made and results were analyzed. PCR assay was compared with bacterial culture method. Results Primer P7/P8 was the most sensitive in four and the sensitivity of this test could reach 0.01 pg of baterial DNA. PCR detecting percentage in animal colony positive for Helicobacter spp. was 80% - 100% and 40% - 50% could be attained in bacterial culture method. There were almost no differences in detecting percentages of cecum and colon. Conclusions PCR assay using P7/P8 could be chosen to preliminarily detect rodent Helicobacter and bacterial culture method could be used to validate the results of PCR, sampling site could be selected in cecum or colon, as well.

关 键 词：啮齿类动物 螺杆菌 检测 

分 类 号：R-33[医药卫生]