大肠癌组织中COX-2的检测及其临床意义  

Expression of cox-2 in human colorectal carcinoma and its clinical significance

作　　者：徐卫国[1] 宋纪宁[1] 杨小青[1] 郝世柱[1] 胡潺潺[1] 李明[1] 吴春涛[1] 朱红成[1] 李瑞伟[1] 赵光明[1] 

机构地区：[1]华北煤炭医学院附属医院肿瘤外科,河北省唐山市063000

出　　处：《中国综合临床》2008年第5期436-438,共3页Clinical Medicine of China

基　　金：河北省唐山市科学技术与发展研究计划资助项目（05134601B）

摘　　要：目的探讨COX-2的表达与肿瘤血管密度（MVD）值及临床病理特征的关系，以揭示COX-2在肿瘤的形成、发展和新生血管形成过程中所发挥的作用。方法采用免疫组织化学法和RT—PCR法测定45例标本的COX-2表达水平，采用CD105标记肿瘤组织中的新生血管计算MVD值，分析COX-2、MVD值与大肠癌生物学性质的关系，分析COX-2表达与MVD的相关性。结果COX-2 mRNA和蛋白的表达在肿瘤是否有淋巴结转移，Dukes分期和肿瘤的不同分化程度各个分组之间差异有统计学意义（均P〈0.05）。MVD值在肿瘤是否有淋巴结转移、Dukes分期、浸润深度各个分组之间差异均有统计学意义（均P〈0．05）。大肠癌中COX-2在蛋白水平和基因转录水平的表达均与MVD值有正相关（P〈0．05）。结论COX-2可能在大肠癌的发生和发展中起重要作用，COX-2促进新生血管的生成，从而促进大肠癌的浸润和转移。Objective To investigate whether cyclooxygenase-2 （COX-2） expression are related to the clinicopathological features,and to microvessel density （ MVD ）. Methods COX-2 expressions were detected in 45 colorectal carcinoma samples by immunostaining. MVD was evaluated with the specific antibody CD105. Quantitation of COX-2 mRNA was performed by RT -PCR in 45 specimens of Colorectal carcinoma. Results The expressions of COX-2 mRNA and protein were significant variation among lymphatic metastasis, high Dukes stages and grade of differation （ P 〈 0.05 ） ; Increased value of MVD were significant variation among lymphatic metastasis, high Dukes stage and depth of tumor invasion （ P 〈 0.05 ）. Both the overexpression of COX-2 protein and mRNA was positively correlated with the value of MVD （ P 〈 0.05 ）. Conelusion COX-2 plays an important role in the development of colorectal carcinoma. COX-2 may up-regulate the value of MVD,which induces angiogenesis and accordingly contributes to metastasis.

关 键 词：大肠癌 COX-2 CD105 肿瘤血管密度 新生血管形成 

分 类 号：R735.34[医药卫生—肿瘤]