TGF-β1在复合异种部分脱钙骨修复兔下颌骨缺损中表达的实验研究  

EMPIRICAL STUDY ON THE EXPRESSION OF TGF-β1 COMPOUND WITH PDXB TO RESTORE RABBIT'S MANDIBULAR DEFECT

作　　者：杨晓辉[1] 钟明红[1] 金耀清[1] 许彪[2] 范红渠 

机构地区：[1]浙江省中医院,杭州310006 [2]昆明医学院附属口腔医院 [3]东风公司襄樊医院

出　　处：《中医正骨》2008年第5期3-5,共3页The Journal of Traditional Chinese Orthopedics and Traumatology

摘　　要：目的：观察不同浓度碱性成纤维细胞生长因子（bFGF）复合并种部分脱钙骨（PDXB）修复兔下颌骨缺损时转化生长因子－β1（TGF-131）的表达情况，分析bFGF浓度与TGF－β1蛋白表达水平之间的相互关系。方法：将54只成年新西兰兔双侧下颌骨下缘形成15mm×15mm全层骨膜骨质缺损，分别植入梯度浓度（1—100ng·mm^-3）PDXB／bFGF混合充填材料和单纯异种部分脱钙骨，术后2周、4周和8周取材行HE染色及免疫组化染色，观察TGF－β1蛋白表达情况并量化计算TGF－β1蛋白表达水平。结果：TGF－β1蛋白表达水平与bFGF的复合浓度和植入时间具有密切关系：在10ng·mm^-3浓度时，TGF－β1蛋白表达水平最强；术后2～4周，TGF－β蛋白呈强阳性表达，而在术后4～8周，TGF－β1蛋白表达呈现明显递减趋势。结论：bFGF复合异种部分脱钙骨可显著地上调骨移植区TGF－β1的表达，复合bFGF浓度为10ng·mm^-3时促进作用最强。Objective:To observe the expression of transforming growth factor-β1 (TGF-β1) when different levels of basic fibroblast growth factor (bPGF)compounded with partly demineralized xenogeneic bone (PDXB) was used to restore rabbit's mandibular defect, and analyze the correlations of bFGF's levels and the expression of TGF-β1. Methods: 54 cases of adult New Zealand rabbit were made models with 15 mm × 15 mm full-thickness deossiflcation of periost on both sides of inferior border of mandible, different grades of mixed filling material (PDXB and bFGF, levels from 1 ng× mm-3 to 100 ng× mm-3) and purely PDXB were implanted respectively, the materials were drawn and made to he staining and immunohistochemical staining after 2 weeks, 4 weeks and 8 weeks. The expressions of TGF-β1 were observed and the levels of them were got quantum chemistry calculation. Result: There had intimate relations of the expressions of TGF-β1 with compound density and implantation times of bFGF. The level on the expressions of TGF-β1 was the best when the density was 10 ng × mm-3. The expressions of TGF-β1 was bettermasculine after 2 ~ 4 weeks and was obviously decrease progressively in the course of 4 weeks to 8 weeks post-operation. Conclusion: The mix of bFGF and PDXB could remarkably up-regulation the expression of TCF-β1 in the area of bone transplantation, and the promotion was best when the density was 10 ng× mm-3.

关 键 词：骨缺损/移植术 混合充填材料/药效学 转化生长因子-β1的表达/药物作用 实验研究 动物 兔 

分 类 号：R782[医药卫生—口腔医学]