外源激素及培养方式对马铃薯试管薯的诱导效应  

Effect of hormone and culture methods on potato microtuber induction

作　　者：杨宏羽[1] 王蒂[1] 潘新 

机构地区：[1]甘肃农业大学农学院,甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [2]敦煌市莫高镇镇政府,甘肃敦煌736200

出　　处：《甘肃农业大学学报》2008年第2期74-77,共4页Journal of Gansu Agricultural University

基　　金：国家863项目(2006AA100107);国家973项目(2006CB-708200);甘肃省农业厅项目(GNSW-2006-01)

摘　　要：以马铃薯普通四倍体栽培品种"抗疫白"和"陇薯3号"脱毒苗为材料,研究了离体条件下外源激素和3种培养方式对马铃薯试管薯诱导和发育的影响.结果表明:外源激素有利于提高试管薯的产量和质量,其诱导效应依次为:6-BA>6-BA+CCC>CCC>对照.激素诱导下3种培养方式对试管薯的诱导效率在单瓶薯数、单瓶薯质量、薯块平均直径及结薯率间差异显著.3种培养方式的诱导效应依次为:液体培养>固液双层培养>固体培养.Potato tetraploid cultivar ＇Kangyibai＇ and ＇Long No. 3＇ were used to investigated the effects of plant hormone and culture methods on induction and development of microtubers in vitro. The results showed that the plant hormone improved the quantity and quality of microbuber and the induction effect could be ordered 6-BA〉6-BA＋CCC〉CCC〉control. Three ways culture included liquid culture,the double layer culture and solid culture were used to examine the differences in the number,weight and average diameter of microtubers per flask. The liquid culture showed the best

关 键 词：马铃薯 试管薯 外源激素 培养方式 

分 类 号：S532.03[农业科学—作物学]