Cre基因拼接及转基因细胞的构建  

Gene splicing of Cre and Construction of Transgenic Cell

作　　者：毛丹[1] 逄大欣 聂代邦[2] 吴婷婷 欧阳红生[2] 

机构地区：[1]吉林大学,130062 [2]江西农业大学,330045

出　　处：《中国科技信息》2008年第11期210-211,共2页China Science and Technology Information

摘　　要：由于具有时间、组织和位点特异性,Cre重组酶介导的DNA重组已成为基因靶位操作的一个重要工具[1]。本实验人工合成Cre基因片段,并将其准确拼接,然后在动物(猪)的成纤维细胞中表达,为接下来构建含Cre基因的转基因猪奠定基础。DNA recombination mediated by cre recombinase has become an important tool for gene targeting because it is time-specific, tissue-specific and site specific. The objective of this study was to splice the Cre gene and to express in mammalian ceils to examine whether the Cre gene can be functionally expressed in mammalian ceils in a high efficiency way. The study is a fundamental one which provides a basis for future research.

关 键 词：CRE基因 基因拼接 猪胚胎成纤维细胞 

分 类 号：R346[医药卫生—基础医学]